Real-time dynamics of Plasmodium NDC80 reveals unusual modes of chromosome segregation during parasite proliferation.
- Zeeshan M School of Life Sciences, Queen's Medical Centre, University of Nottingham, Nottingham NG7 2UH, UK.
- Pandey R School of Life Sciences, Queen's Medical Centre, University of Nottingham, Nottingham NG7 2UH, UK.
- Ferguson DJP Nuffield Department of Clinical Laboratory Science, University of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, UK.
- Tromer EC Department of Biochemistry, University of Cambridge, Cambridge CB2 1QW, UK.
- Markus R School of Life Sciences, Queen's Medical Centre, University of Nottingham, Nottingham NG7 2UH, UK.
- Abel S Department of Molecular, Cell and Systems Biology, University of California Riverside, Riverside, CA 92521, USA.
- Brady D School of Life Sciences, Queen's Medical Centre, University of Nottingham, Nottingham NG7 2UH, UK.
- Daniel E School of Life Sciences, Queen's Medical Centre, University of Nottingham, Nottingham NG7 2UH, UK.
- Limenitakis R Institute of Cell Biology, University of Bern, Bern 3012, Switzerland.
- Bottrill AR School of Life Sciences, Gibbelt Hill Campus, University of Warwick, Coventry CV4 7AL, UK.
- Le Roch KG Department of Molecular, Cell and Systems Biology, University of California Riverside, Riverside, CA 92521, USA.
- Holder AA Malaria Parasitology Laboratory, The Francis Crick Institute, London NW1 1AT, UK.
- Waller RF Department of Biochemistry, University of Cambridge, Cambridge CB2 1QW, UK.
- Guttery DS Leicester Cancer Research Centre, University of Leicester, Leicester LE2 7LX, UK.
- Tewari R School of Life Sciences, Queen's Medical Centre, University of Nottingham, Nottingham NG7 2UH, UK rita.tewari@nottingham.ac.uk.
- 2020-06-06
Published in:
- Journal of cell science. - 2020
Atypical cell division
Chromosome segregation
Endomitosis
Kinetochore
Malaria
Meiosis
NDC80 complex
Plasmodium
English
Eukaryotic cell proliferation requires chromosome replication and precise segregation to ensure daughter cells have identical genomic copies. Species of the genus Plasmodium, the causative agents of malaria, display remarkable aspects of nuclear division throughout their life cycle to meet some peculiar and unique challenges to DNA replication and chromosome segregation. The parasite undergoes atypical endomitosis and endoreduplication with an intact nuclear membrane and intranuclear mitotic spindle. To understand these diverse modes of Plasmodium cell division, we have studied the behaviour and composition of the outer kinetochore NDC80 complex, a key part of the mitotic apparatus that attaches the centromere of chromosomes to microtubules of the mitotic spindle. Using NDC80-GFP live-cell imaging in Plasmodium berghei, we observe dynamic spatiotemporal changes during proliferation, including highly unusual kinetochore arrangements during sexual stages. We identify a very divergent candidate for the SPC24 subunit of the NDC80 complex, previously thought to be missing in Plasmodium, which completes a canonical, albeit unusual, NDC80 complex structure. Altogether, our studies reveal the kinetochore to be an ideal tool to investigate the non-canonical modes of chromosome segregation and cell division in Plasmodium.
- Language
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- English
- Open access status
- hybrid
- Identifiers
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- DOI 10.1242/jcs.245753
- PMID 32501284
- Persistent URL
- https://sonar.ch/global/documents/194
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