Analysis of mtDNA/nDNA Ratio in Mice.
- Quiros PM Laboratory for Integrative and Systems Physiology, École Polytechnique Federale de Lausanne, Lausanne, Switzerland.
- Goyal A Laboratory for Integrative and Systems Physiology, École Polytechnique Federale de Lausanne, Lausanne, Switzerland.
- Jha P Laboratory for Integrative and Systems Physiology, École Polytechnique Federale de Lausanne, Lausanne, Switzerland.
- Auwerx J Laboratory for Integrative and Systems Physiology, École Polytechnique Federale de Lausanne, Lausanne, Switzerland.
- 2017-03-03
Published in:
- Current protocols in mouse biology. - 2017
16SrRNA
ND1
mtDNA
qPCR
Age Factors
Animals
Cell Nucleus
DNA
DNA, Mitochondrial
Gene Dosage
Genes, Mitochondrial
Mice
Polymerase Chain Reaction
Reproducibility of Results
English
Mitochondrial DNA (mtDNA) lacks the protection provided by the nucleosomes in the nuclear DNA and does not have a DNA repair mechanism, making it highly susceptible to damage, which can lead to mtDNA depletion. mtDNA depletion compromises the efficient function of cells and tissues and thus impacts negatively on health. Here, we describe a brief and easy protocol to quantify mtDNA copy number by determining the mtDNA/nDNA ratio. The procedure has been validated using a cohort of young and aged mice. © 2017 by John Wiley & Sons, Inc.
- Language
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- English
- Open access status
- green
- Identifiers
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- DOI 10.1002/cpmo.21
- PMID 28252199
- Persistent URL
- https://sonar.ch/global/documents/194184
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