Phenalen-1-one-Mediated Antimicrobial Photodynamic Therapy: Antimicrobial Efficacy in a Periodontal Biofilm Model and Flow Cytometric Evaluation of Cytoplasmic Membrane Damage.

Cieplik F; Steinwachs VS; Muehler D; Hiller KA; Thurnheer T; Belibasakis GN; Buchalla W; Maisch T

doi:10.3389/fmicb.2018.00688

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Journal article

Phenalen-1-one-Mediated Antimicrobial Photodynamic Therapy: Antimicrobial Efficacy in a Periodontal Biofilm Model and Flow Cytometric Evaluation of Cytoplasmic Membrane Damage.

Cieplik F Department of Conservative Dentistry and Periodontology, University Medical Center Regensburg, Regensburg, Germany.
Steinwachs VS Department of Conservative Dentistry and Periodontology, University Medical Center Regensburg, Regensburg, Germany.
Muehler D Department of Conservative Dentistry and Periodontology, University Medical Center Regensburg, Regensburg, Germany.
Hiller KA Department of Conservative Dentistry and Periodontology, University Medical Center Regensburg, Regensburg, Germany.
Thurnheer T Division of Oral Microbiology and Immunology, Clinic of Preventive Dentistry, Periodontology and Cariology, Center of Dental Medicine, University of Zurich, Zurich, Switzerland.
Belibasakis GN Division of Oral Diseases, Department of Dental Medicine, Karolinska Institutet, Solna, Sweden.
Buchalla W Department of Conservative Dentistry and Periodontology, University Medical Center Regensburg, Regensburg, Germany.
Maisch T Department of Dermatology, University Medical Center Regensburg, Regensburg, Germany.

2018-04-24

Published in:

Frontiers in microbiology. - 2018

English In light of increasing resistance toward conventional antibiotics and antiseptics, antimicrobial photodynamic therapy (aPDT) may be a valuable alternative, especially for use in dentistry. In this regard, photosensitizers (PS) based on a phenalen-1-one structure seem to be especially favorable due to their high singlet oxygen quantum yield. However, the actual target structures of phenalen-1-one-mediated aPDT are still unclear. The aim of the present study was to investigate the antimicrobial efficacy of aPDT mediated by phenalen-1-one derivatives SAPYR and SAGUA for inactivation of a polymicrobial biofilm consisting of three putative periodontal pathogens in vitro and to get first insights in the mechanism of action of phenalen-1-one-mediated aPDT by assessing damage of cytoplasmic membranes. aPDT with SAPYR exhibited identical antimicrobial efficacy as compared to chlorhexidine (CHX) [4.4-6.1 log10 reduction of colony forming units (CFUs) depending on bacterial species] while aPDT with SAGUA was less effective (2.0-2.8 log10). Flow cytometric analysis combined with propidium iodide (PI) staining revealed no damage of cytoplasmic membranes after aPDT with both phenalen-1-one derivatives, which was confirmed by spectroscopic measurements for release of nucleic acids after treatment. Spectrophotometric PS-uptake measurements showed no uptake of SAPYR by bacterial cells. Despite the inability to pinpoint the actual target of phenalen-1-one-mediated aPDT, this study shows the high antimicrobial potential of phenalen-1-on mediated aPDT (especially when using SAPYR) and represents a first step for getting insights in the mechanism and damage patterns of aPDT with this class of PS.

Language

English

Open access status

gold

Identifiers

DOI 10.3389/fmicb.2018.00688
PMID 29681899

Persistent URL

https://sonar.ch/global/documents/195291

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