Journal article
The Sendai virus nonstructural C proteins specifically inhibit viral mRNA synthesis.
- Curran J Department of Genetics and Microbiology, University of Geneva School of Medicine, Switzerland.
- Marq JB
- Kolakofsky D
- 1992-08-01
Published in:
- Virology. - 1992
Base Sequence
Cells, Cultured
Gene Expression Regulation, Viral
In Vitro Techniques
Molecular Sequence Data
Mutagenesis, Site-Directed
Oligodeoxyribonucleotides
Parainfluenza Virus 1, Human
RNA, Messenger
RNA, Viral
Transcription, Genetic
Viral Proteins
English
An in vitro transcription system for paramyxoviruses is described, in which polymerase-free templates are combined with cell extracts containing polymerase made in vivo via transfected plasmids. Both P and L are required for polymerase activity, and both must be coexpressed for optimum activity. mRNA synthesis here was found to be inversely proportional to the level of C expression, whereas defective interfering genome replication was largely unaffected by the level of C in the extract. The inhibition of transcription appeared to be due to the C' and C, but not the Y1 and Y2 proteins, and only occurred when C'/C was coexpressed with P and L. C'/C appears to intervene during polymerase formation, possibly by forming polymerase complexes which are inactive for transcription, but still competent for genome replication.
- Language
-
- English
- Open access status
- closed
- Identifiers
-
- DOI 10.1016/0042-6822(92)90588-g
- PMID 1322593
- Persistent URL
- https://sonar.ch/global/documents/195612
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