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Protein (western) blotting.
Journal article

Protein (western) blotting.

  • Egger D Institute for Medical Microbiology, University of Basel, Switzerland.
  • Bienz K
  • 1994-06-01
Published in:
  • Molecular biotechnology. - 1994
Antibodies
Biotechnology
Blotting, Western
Buffers
DNA-Binding Proteins
Electrophoresis, Polyacrylamide Gel
Membranes, Artificial
Proteins
RNA-Binding Proteins
Sensitivity and Specificity
Staining and Labeling
English The different steps involved in protein (Western) blotting and subsequent analysis of the proteins are reviewed. Electrophoretic separation of proteins, procedures of transfer to membranes, immunological and nonimmunological protein detection systems, and characterization of protein-nucleic acid and protein-protein interactions are described. Emphasis is on the sensitivity of the methods described and on possible variations that allow the individual steps of Western blotting to be adapted to specific questions.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/198145
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