Correlation of photodynamic activity and fluorescence signaling for free and pegylated mTHPC in mesothelioma xenografts.
- Tran N Division of Thoracic Surgery, University of Lausanne, Lausanne, Switzerland.
- Krueger T
- Pan Y
- Yan H
- Cheng C
- Altermatt HJ
- Ballini JP
- Borle F
- Ris HB
- Andrejevic-Blant S
- 2007-03-09
Published in:
- Lasers in surgery and medicine. - 2007
Animals
Female
Mesoporphyrins
Mesothelioma
Mice
Mice, Nude
Microscopy, Fluorescence
Necrosis
Neoplasm Transplantation
Photochemotherapy
Photosensitizing Agents
Pleural Neoplasms
Transplantation, Heterologous
English
BACKGROUND/OBJECTIVES
Correlation of photodynamic activity (PDT) and fluorescence signaling for free and pegylated meta-tetrahydroxyphenylchlorin (mTHPC) in nude mice with mesothelioma xenografts.
STUDY DESIGN/MATERIALS AND METHODS
Twelve animals received light delivery (20 J/cm(2), 150 mW/cm(2), spot size 1.2 cm) on the tumor and the hind leg 3 days after sensitization with 0.15 mg/kg free mTHPC (n = 6) or equimolar-dosed pegylated mTHPC (n = 6). Groups of three animals each were sensitized with 0.15 and 0.5 mg/kg free mTHPC or equimolar dosed pegylated mTHPC followed after 3 days by fluorescence microscopy measurements.
RESULTS
Pegylated mTHPC resulted in a similar extent of PDT-related tumor necrosis but in lower skin phototoxicity than free mTHPC. Both mTHPC formulations were heterogeneously distributed in the tumor and were mainly localized in perivascular areas. Pegylated mTHPC revealed a higher tumor to skin fluorescence intensity ratio than free mTHPC (P<0.001).
CONCLUSIONS
Fluorescence signaling measurement has the potential to predict the photodynamic activity for both mTHPC formulations in mesothelioma xenografts.
Correlation of photodynamic activity (PDT) and fluorescence signaling for free and pegylated meta-tetrahydroxyphenylchlorin (mTHPC) in nude mice with mesothelioma xenografts.
STUDY DESIGN/MATERIALS AND METHODS
Twelve animals received light delivery (20 J/cm(2), 150 mW/cm(2), spot size 1.2 cm) on the tumor and the hind leg 3 days after sensitization with 0.15 mg/kg free mTHPC (n = 6) or equimolar-dosed pegylated mTHPC (n = 6). Groups of three animals each were sensitized with 0.15 and 0.5 mg/kg free mTHPC or equimolar dosed pegylated mTHPC followed after 3 days by fluorescence microscopy measurements.
RESULTS
Pegylated mTHPC resulted in a similar extent of PDT-related tumor necrosis but in lower skin phototoxicity than free mTHPC. Both mTHPC formulations were heterogeneously distributed in the tumor and were mainly localized in perivascular areas. Pegylated mTHPC revealed a higher tumor to skin fluorescence intensity ratio than free mTHPC (P<0.001).
CONCLUSIONS
Fluorescence signaling measurement has the potential to predict the photodynamic activity for both mTHPC formulations in mesothelioma xenografts.
- Language
-
- English
- Open access status
- green
- Identifiers
-
- DOI 10.1002/lsm.20477
- PMID 17345623
- Persistent URL
- https://sonar.ch/global/documents/19936
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