Journal article

Single-bacterial genomics validates rich and varied specialized metabolism of uncultivated Entotheonella sponge symbionts.

  • Mori T Department of Life Science and Medical Biosciences, Waseda University, Shinjuku-ku, 162-8480 Tokyo, Japan.
  • Cahn JKB Institute of Microbiology, Eidgenössische Technische Hochschule (ETH) Zürich, 8093 Zürich, Switzerland.
  • Wilson MC Institute of Microbiology, Eidgenössische Technische Hochschule (ETH) Zürich, 8093 Zürich, Switzerland.
  • Meoded RA Institute of Microbiology, Eidgenössische Technische Hochschule (ETH) Zürich, 8093 Zürich, Switzerland.
  • Wiebach V Institute of Microbiology, Eidgenössische Technische Hochschule (ETH) Zürich, 8093 Zürich, Switzerland.
  • Martinez AFC Institute of Microbiology, Eidgenössische Technische Hochschule (ETH) Zürich, 8093 Zürich, Switzerland.
  • Helfrich EJN Institute of Microbiology, Eidgenössische Technische Hochschule (ETH) Zürich, 8093 Zürich, Switzerland.
  • Albersmeier A Center for Biotechnology, Universität Bielefeld, 33615 Bielefeld, Germany.
  • Wibberg D Center for Biotechnology, Universität Bielefeld, 33615 Bielefeld, Germany.
  • Dätwyler S Institute of Microbiology, Eidgenössische Technische Hochschule (ETH) Zürich, 8093 Zürich, Switzerland.
  • Keren R Department of Zoology, George S. Wise Faculty of Life Sciences, Tel Aviv University, 69978 Tel Aviv, Israel.
  • Lavy A Department of Zoology, George S. Wise Faculty of Life Sciences, Tel Aviv University, 69978 Tel Aviv, Israel.
  • Rückert C Center for Biotechnology, Universität Bielefeld, 33615 Bielefeld, Germany.
  • Ilan M Department of Zoology, George S. Wise Faculty of Life Sciences, Tel Aviv University, 69978 Tel Aviv, Israel.
  • Kalinowski J Center for Biotechnology, Universität Bielefeld, 33615 Bielefeld, Germany.
  • Matsunaga S Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, 113-8657 Tokyo, Japan.
  • Takeyama H Department of Life Science and Medical Biosciences, Waseda University, Shinjuku-ku, 162-8480 Tokyo, Japan.
  • Piel J Institute of Microbiology, Eidgenössische Technische Hochschule (ETH) Zürich, 8093 Zürich, Switzerland; jpiel@ethz.ch.
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  • 2018-02-14
Published in:
  • Proceedings of the National Academy of Sciences of the United States of America. - 2018
English Marine sponges are prolific sources of unique bioactive natural products. The sponge Theonella swinhoei is represented by several distinct variants with largely nonoverlapping chemistry. For the Japanese chemotype Y harboring diverse complex polyketides and peptides, we previously provided genomic and functional evidence that a single symbiont, the filamentous, multicellular organism "Candidatus Entotheonella factor," produces almost all of these compounds. To obtain further insights into the chemistry of "Entotheonella," we investigated another phylotype, "Candidatus Entotheonella serta," present in the T. swinhoei WA sponge chemotype, a source of theonellamide- and misakinolide-type compounds. Unexpectedly, considering the lower chemical diversity, sequencing of individual bacterial filaments revealed an even larger number of biosynthetic gene regions than for Ca E. factor, with virtually no overlap. These included genes for misakinolide and theonellamide biosynthesis, the latter assigned by comparative genomic and metabolic analysis of a T. swinhoei chemotype from Israel, and by biochemical studies. The data suggest that both compound families, which were among the earliest model substances to study bacterial producers in sponges, originate from the same bacterium in T. swinhoei WA. They also add evidence that metabolic richness and variability could be a more general feature of Entotheonella symbionts.
Language
  • English
Open access status
bronze
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Persistent URL
https://sonar.ch/global/documents/199521
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