Back
Full-text
Journal article

Reporting Guidelines, Review of Methodological Standards, and Challenges Toward Harmonization in Bone Marrow Adiposity Research. Report of the Methodologies Working Group of the International Bone Marrow Adiposity Society.

  • Tratwal J Laboratory of Regenerative Hematopoiesis, Institute of Bioengineering and Swiss Institute for Experimental Cancer Research, Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.
  • Labella R Tissue and Tumour Microenvironments Lab, The Kennedy Institute of Rheumatology, University of Oxford, Oxford, United Kingdom.
  • Bravenboer N Department of Clinical Chemistry, Amsterdam University Medical Centers, Vrije Universiteit, Amsterdam Movement Sciences, Amsterdam, Netherlands.
  • Kerckhofs G Biomechanics Lab, Institute of Mechanics, Materials and Civil Engineering, UCLouvain, Louvain-la-Neuve, Belgium.
  • Douni E Laboratory of Genetics, Department of Biotechnology, Agricultural University of Athens, Athens, Greece.
  • Scheller EL Division of Bone and Mineral Diseases, Department of Medicine, Washington University, St. Louis, MO, United States.
  • Badr S Univ. Lille, EA 4490 - PMOI - Physiopathologie des Maladies Osseuses Inflammatoires, Lille, France.
  • Karampinos DC Department of Diagnostic and Interventional Radiology, Technical University of Munich, Munich, Germany.
  • Beck-Cormier S Inserm, UMR 1229, RMeS, Regenerative Medicine and Skeleton, Université de Nantes, ONIRIS, Nantes, France.
  • Palmisano B Department of Genetics and Development, Columbia University Irving Medical Center, New York, NY, United States.
  • Poloni A Hematology, Department of Clinic and Molecular Science, Università Politecnica Marche-AOU Ospedali Riuniti, Ancona, Italy.
  • Moreno-Aliaga MJ Centre for Nutrition Research and Department of Nutrition, Food Science and Physiology, School of Pharmacy and Nutrition, University of Navarra, Pamplona, Spain.
  • Fretz J Department of Orthopaedics and Rehabilitation, Cellular and Developmental Biology, Yale University School of Medicine, New Haven, CT, United States.
  • Rodeheffer MS Department of Comparative Medicine and Molecular, Cellular and Developmental Biology, Yale University School of Medicine, New Haven, CT, United States.
  • Boroumand P Cell Biology Program, The Hospital for Sick Children, Toronto, ON, Canada.
  • Rosen CJ Maine Medical Center Research Institute, Center for Clinical and Translational Research, Scarborough, ME, United States.
  • Horowitz MC Department of Orthopaedics and Rehabilitation, Yale University School of Medicine, New Haven, CT, United States.
  • van der Eerden BCJ Laboratory for Calcium and Bone Metabolism, Department of Internal Medicine, Erasmus University Medical Center, Rotterdam, Netherlands.
  • Veldhuis-Vlug AG Section of Endocrinology, Department of Internal Medicine, Center for Bone Quality, Leiden University Medical Center, Leiden, Netherlands.
  • Naveiras O Laboratory of Regenerative Hematopoiesis, Institute of Bioengineering and Swiss Institute for Experimental Cancer Research, Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.
Show more…
  • 2020-03-18
Published in:
  • Frontiers in endocrinology. - 2020
Bone Marrow Adiposity Society
adipocyte
bone marrow adipose tissue
bone marrow adiposity
bone marrow fat
marrow
methods
standards
English The interest in bone marrow adiposity (BMA) has increased over the last decade due to its association with, and potential role, in a range of diseases (osteoporosis, diabetes, anorexia, cancer) as well as treatments (corticosteroid, radiation, chemotherapy, thiazolidinediones). However, to advance the field of BMA research, standardization of methods is desirable to increase comparability of study outcomes and foster collaboration. Therefore, at the 2017 annual BMA meeting, the International Bone Marrow Adiposity Society (BMAS) founded a working group to evaluate methodologies in BMA research. All BMAS members could volunteer to participate. The working group members, who are all active preclinical or clinical BMA researchers, searched the literature for articles investigating BMA and discussed the results during personal and telephone conferences. According to the consensus opinion, both based on the review of the literature and on expert opinion, we describe existing methodologies and discuss the challenges and future directions for (1) histomorphometry of bone marrow adipocytes, (2) ex vivo BMA imaging, (3) in vivo BMA imaging, (4) cell isolation, culture, differentiation and in vitro modulation of primary bone marrow adipocytes and bone marrow stromal cell precursors, (5) lineage tracing and in vivo BMA modulation, and (6) BMA biobanking. We identify as accepted standards in BMA research: manual histomorphometry and osmium tetroxide 3D contrast-enhanced μCT for ex vivo quantification, specific MRI sequences (WFI and H-MRS) for in vivo studies, and RT-qPCR with a minimal four gene panel or lipid-based assays for in vitro quantification of bone marrow adipogenesis. Emerging techniques are described which may soon come to complement or substitute these gold standards. Known confounding factors and minimal reporting standards are presented, and their use is encouraged to facilitate comparison across studies. In conclusion, specific BMA methodologies have been developed. However, important challenges remain. In particular, we advocate for the harmonization of methodologies, the precise reporting of known confounding factors, and the identification of methods to modulate BMA independently from other tissues. Wider use of existing animal models with impaired BMA production (e.g., Pfrt -/-, KitW/W-v) and development of specific BMA deletion models would be highly desirable for this purpose.
Language
  • English
Open access status
gold
Identifiers
Persistent URL
https://sonar.ch/global/documents/201103
Statistics
Document views: 59 File downloads:
  • Full-text: 0