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Probing the interactions of NK cell receptors with ligand expressed in trans and cis.
Journal article

Probing the interactions of NK cell receptors with ligand expressed in trans and cis.

  • Back J Ludwig Institute for Cancer Research Ltd., Lausanne Branch, and University of Lausanne, Epalinges, Switzerland.
  • Scarpellino L
  • Held W
  • 2009-12-25
Published in:
  • Methods in molecular biology (Clifton, N.J.). - 2010
Animals
Cell Adhesion
Cell Line
Cell Membrane
Gene Expression Regulation
Killer Cells, Natural
Ligands
NK Cell Lectin-Like Receptor Subfamily A
Protein Binding
Solubility
Substrate Specificity
English Certain receptors on natural killer (NK) cells, which are specific for MHC class I (MHC-I) molecules, do not only interact with ligand expressed on opposing cell membranes (in trans) but also interact with those on the same cell membrane (in cis). Cis interactions have been demonstrated for only a small number of cell surface receptors. However, this has not been tested systematically, raising the possibility that additional receptors may be able to bind ligand expressed in cis. Here we describe a number of approaches to evaluate trans and cis binding of the Ly49A NK cell receptor to its H-2D(d) ligand. These procedures should facilitate the investigation of cis/trans interactions of other receptor-ligand pairs and simplify the analysis of NK cell receptor variants.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/20151
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