AmotL2 links VE-cadherin to contractile actin fibres necessary for aortic lumen expansion.
- Hultin S Department of Oncology and Pathology, Cancer Center Karolinska, Karolinska University Hospital, 171 76 Stockholm, Sweden.
- Zheng Y Department of Oncology and Pathology, Cancer Center Karolinska, Karolinska University Hospital, 171 76 Stockholm, Sweden.
- Mojallal M Department of Oncology and Pathology, Cancer Center Karolinska, Karolinska University Hospital, 171 76 Stockholm, Sweden.
- Vertuani S Department of Oncology and Pathology, Cancer Center Karolinska, Karolinska University Hospital, 171 76 Stockholm, Sweden.
- Gentili C Department of Oncology and Pathology, Cancer Center Karolinska, Karolinska University Hospital, 171 76 Stockholm, Sweden.
- Balland M Laboratoire interdisciplinaire de Physique, Université Joseph Fourier (Grenoble 1), Domaine Universitaire, Bat. E45 140, rue de la physique, Saint Martin d´Hères Cedex 9 38402 Grenoble, France.
- Milloud R Laboratoire interdisciplinaire de Physique, Université Joseph Fourier (Grenoble 1), Domaine Universitaire, Bat. E45 140, rue de la physique, Saint Martin d´Hères Cedex 9 38402 Grenoble, France.
- Belting HG Abt. Zellbiologie, Biozentrum/Uni Basel, Klingelbergstrasse 70, CH 4056 Basel, Switzerland.
- Affolter M Abt. Zellbiologie, Biozentrum/Uni Basel, Klingelbergstrasse 70, CH 4056 Basel, Switzerland.
- Helker CS Biological Faculty, University of Muenster, 48149 Muenster, Germany.
- Adams RH Max Planck Institute for Molecular Biomedicine, 48149 Muenster, Germany.
- Herzog W 1] Biological Faculty, University of Muenster, 48149 Muenster, Germany [2] Max Planck Institute for Molecular Biomedicine, 48149 Muenster, Germany [3] Cluster of Excellence EXC 1003, Cells in Motion, CiM -, 48149 Münster, Germany.
- Uhlen P Department of Medical Biochemistry and Biophysics, Karolinska Institute, 171 77 Stockholm, Sweden.
- Majumdar A 1] Department of Oncology and Pathology, Cancer Center Karolinska, Karolinska University Hospital, 171 76 Stockholm, Sweden [2].
- Holmgren L 1] Department of Oncology and Pathology, Cancer Center Karolinska, Karolinska University Hospital, 171 76 Stockholm, Sweden [2].
- 2014-05-09
Published in:
- Nature communications. - 2014
Actin Cytoskeleton
Adherens Junctions
Animals
Antigens, CD
Aorta
Cadherins
Cell Communication
Cell Shape
Contractile Proteins
Endothelial Cells
Endothelium, Vascular
Extracellular Matrix
Gene Knockdown Techniques
Membrane Proteins
Mice
Mice, Inbred C57BL
Mice, Knockout
Morphogenesis
Morpholinos
Neovascularization, Physiologic
RNA Interference
RNA, Small Interfering
Zebrafish
Zebrafish Proteins
English
The assembly of individual endothelial cells into multicellular tubes is a complex morphogenetic event in vascular development. Extracellular matrix cues and cell-cell junctional communication are fundamental to tube formation. Together they determine the shape of endothelial cells and the tubular structures that they ultimately form. Little is known regarding how mechanical signals are transmitted between cells to control cell shape changes during morphogenesis. Here we provide evidence that the scaffold protein amotL2 is needed for aortic vessel lumen expansion. Using gene inactivation strategies in zebrafish, mouse and endothelial cell culture systems, we show that amotL2 associates to the VE-cadherin adhesion complex where it couples adherens junctions to contractile actin fibres. Inactivation of amotL2 dissociates VE-cadherin from cytoskeletal tensile forces that affect endothelial cell shape. We propose that the VE-cadherin/amotL2 complex is responsible for transmitting mechanical force between endothelial cells for the coordination of cellular morphogenesis consistent with aortic lumen expansion and function.
- Language
-
- English
- Open access status
- bronze
- Identifiers
-
- DOI 10.1038/ncomms4743
- PMID 24806444
- Persistent URL
- https://sonar.ch/global/documents/202316
Export to…
Statistics
Document views: 79
File downloads:
- Full-text: 0