Back
The PIAS homologue Siz2 regulates perinuclear telomere position and telomerase activity in budding yeast.
Journal article

The PIAS homologue Siz2 regulates perinuclear telomere position and telomerase activity in budding yeast.

Show more…
  • 2011-06-14
Published in:
  • Nature cell biology. - 2011
DNA Helicases
DNA-Binding Proteins
Microscopy, Fluorescence
Mutation
Nuclear Envelope
Protein Processing, Post-Translational
Recombinant Fusion Proteins
Saccharomyces cerevisiae
Saccharomyces cerevisiae Proteins
Silent Information Regulator Proteins, Saccharomyces cerevisiae
Sumoylation
Telomerase
Telomere
Time Factors
English Budding yeast telomeres are reversibly bound at the nuclear envelope through two partially redundant pathways that involve the Sir2/3/4 silencing complex and the Yku70/80 heterodimer. To better understand how this is regulated, we studied the role of SUMOylation in telomere anchoring. We find that the PIAS-like SUMO E3 ligase Siz2 sumoylates both Yku70/80 and Sir4 in vivo and promotes telomere anchoring to the nuclear envelope. Remarkably, loss of Siz2 also provokes telomere extension in a telomerase-dependent manner that is epistatic with loss of the helicase Pif1. Consistent with our previously documented role for telomerase in anchorage, normal telomere anchoring in siz2 Δ is restored by PIF1 deletion. By live-cell imaging of a critically short telomere, we show that telomeres shift away from the nuclear envelope when elongating. We propose that SUMO-dependent association with the nuclear periphery restrains bound telomerase, whereas active elongation correlates with telomere release.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/205126
Statistics
Document views: 20 File downloads: