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Highly sensitive detection of pharmaceutical compounds in biological fluids using capillary electrophoresis coupled with laser-induced native fluorescence.
Journal article

Highly sensitive detection of pharmaceutical compounds in biological fluids using capillary electrophoresis coupled with laser-induced native fluorescence.

  • Schappler J Laboratory of Pharmaceutical Analytical Chemistry, School of Pharmaceutical Sciences - EPGL, University of Geneva, University of Lausanne, Bd d'Yvoy 20, 1211 Geneva 4, Switzerland.
  • Staub A
  • Veuthey JL
  • Rudaz S
  • 2008-05-13
Published in:
  • Journal of chromatography. A. - 2008
Body Fluids
Electrophoresis, Capillary
Humans
Hydrogen-Ion Concentration
Lasers
Pharmaceutical Preparations
Plasma
Propranolol
Reference Standards
Reproducibility of Results
Sensitivity and Specificity
Spectrometry, Fluorescence
Temperature
English Due to its selectivity and sensitivity, capillary electrophoresis coupled to laser-induced fluorescence has evolved as a useful analytical tool for determining drugs in biological samples. This paper describes a simple, sensitive, efficient, and rapid method for analyzing propranolol without derivatization in plasma by CE-LIF using a diode solid-state laser at 266nm. An experimental design methodology was assessed for the investigation of electrophoretic parameters and a Box-Behnken design was selected to optimize both sensitivity and efficiency. The CE-LIF method was linear over the concentration range of 50-850ngmL(-1) and successfully applied to a real forensic sample.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/205702
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