Inhibition of HIV-1 replication by balsamin, a ribosome inactivating protein of Momordica balsamina.
- Kaur I Department of Microbiology and Molecular Medicine, University of Geneva, Switzerland University of Geneva, Geneva, Switzerland ; Fermentation and Protein Biotechnology Laboratory, Department of Biotechnology, Punjabi University, Patiala, India.
- Puri M
- Ahmed Z
- Blanchet FP
- Mangeat B
- Piguet V
- 2013-09-17
Published in:
- PloS one. - 2013
Animals
CD4-Positive T-Lymphocytes
Cell Line
Cell Line, Tumor
Cell Survival
Cells, Cultured
Dose-Response Relationship, Drug
Drug Resistance, Viral
HIV-1
Host-Pathogen Interactions
Humans
Inhibitory Concentration 50
Jurkat Cells
Momordica
Mutation
Plant Proteins
Ribosome Inactivating Proteins
T-Lymphocytes
Virus Replication
English
Ribosome-inactivating proteins (RIPs) are endowed with several medicinal properties, including antiviral activity. We demonstrate here that the recently identified type I RIP from Momordica balsamina also possesses antiviral activity, as determined by viral growth curve assays and single-round infection experiments. Importantly, this activity is at play even as doses where the RIP has no cytotoxic effect. In addition, balsamin inhibits HIV-1 replication not only in T cell lines but also in human primary CD4(+) T cells. This antiviral compound exerts its activity at a viral replicative step occurring later than reverse-transcription, most likely on viral protein translation, prior to viral budding and release. Finally, we demonstrate that balsamin antiviral activity is broad since it also impedes influenza virus replication. Altogether our results demonstrate that type I RIP can exert a potent anti-HIV-1 activity which paves the way for new therapeutic avenues for the treatment of viral infections.
- Language
-
- English
- Open access status
- gold
- Identifiers
-
- DOI 10.1371/journal.pone.0073780
- PMID 24040067
- Persistent URL
- https://sonar.ch/global/documents/205930
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