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Phospholipid-Block Copolymer Hybrid Vesicles with Lysosomal Escape Ability.
Journal article

Phospholipid-Block Copolymer Hybrid Vesicles with Lysosomal Escape Ability.

  • Zong W State Key Laboratory of Urban Water Resource and Environment, School of Chemistry and Chemical Engineering , Harbin Institute of Technology , 92 West Da-Zhi Street , Harbin 150001 , China.
  • Thingholm B Interdisciplinary Nanoscience Center (iNANO) , Aarhus University , Gustav Wieds Vej 14 , 8000 Aarhus , Denmark.
  • Itel F Interdisciplinary Nanoscience Center (iNANO) , Aarhus University , Gustav Wieds Vej 14 , 8000 Aarhus , Denmark.
  • Schattling PS Interdisciplinary Nanoscience Center (iNANO) , Aarhus University , Gustav Wieds Vej 14 , 8000 Aarhus , Denmark.
  • Brodszkij E Interdisciplinary Nanoscience Center (iNANO) , Aarhus University , Gustav Wieds Vej 14 , 8000 Aarhus , Denmark.
  • Mayer D Institute for Medical Microbiology and Infection Control , University Hospital Ulm , 89021 Ulm , Germany.
  • Stenger S Institute for Medical Microbiology and Infection Control , University Hospital Ulm , 89021 Ulm , Germany.
  • Goldie KN Center for Cellular Imaging & Nano Analytics, Biozentrum , University of Basel , 4056 Basel , Switzerland.
  • Han X State Key Laboratory of Urban Water Resource and Environment, School of Chemistry and Chemical Engineering , Harbin Institute of Technology , 92 West Da-Zhi Street , Harbin 150001 , China.
  • Städler B Interdisciplinary Nanoscience Center (iNANO) , Aarhus University , Gustav Wieds Vej 14 , 8000 Aarhus , Denmark.
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  • 2018-05-20
Published in:
  • Langmuir : the ACS journal of surfaces and colloids. - 2018
Animals
Cell Line
Cell Survival
Drug Delivery Systems
Humans
Mice
Phospholipids
Polymers
Rats
Unilamellar Liposomes
English The success of nanoparticulate formulations in drug delivery depends on various aspects including their toxicity, internalization, and intracellular location. Vesicular assemblies consisting of phospholipids and amphiphilic block copolymers are an emerging platform, which combines the benefits from liposomes and polymersomes while overcoming their challenges. We report the synthesis of poly(cholesteryl methacrylate)- block-poly(2-(dimethylamino) ethyl methacrylate) (pCMA- b-pDMAEMA) block copolymers and their assembly with phospholipids into hybrid vesicles. Their geometry, their ζ-potential, and their ability to adsorb onto polymer-coated surfaces were assessed. Giant unilamellar vesicles were employed to confirm the presence of both the phospholipids and the block copolymer in the same membrane. Furthermore, the cytotoxicity of selected hybrid vesicles was determined in RAW 264.7 mouse macrophages, primary rat Kupffer cells, and human macrophages. The internalization and lysosomal escape ability of the hybrid vesicles were confirmed using RAW 264.7 mouse macrophages. Taken together, our findings illustrate that the reported hybrid vesicles are a promising complementary drug delivery platform for existing liposomes and polymersomes.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/207394
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