Journal article
Self-establishing communities enable cooperative metabolite exchange in a eukaryote
- Campbell, Kate Cambridge Systems Biology Centre, University of Cambridge, Cambridge, United Kingdom
- Vowinckel, Jakob Cambridge Systems Biology Centre, University of Cambridge, Cambridge, United Kingdom
- Mülleder, Michael Cambridge Systems Biology Centre, University of Cambridge, Cambridge, United Kingdom
- Malmsheimer, Silke Cambridge Systems Biology Centre, University of Cambridge, Cambridge, United Kingdom
- Lawrence, Nicola The Wellcome Trust Gurdon Institute, University of Cambridge, Cambridge, United Kingdom
- Calvani, Enrica Cambridge Systems Biology Centre, University of Cambridge, Cambridge, United Kingdom
- Miller-Fleming, Leonor Cambridge Systems Biology Centre, University of Cambridge, Cambridge, United Kingdom
- Alam, Mohammad T Cambridge Systems Biology Centre, University of Cambridge, Cambridge, United Kingdom
- Christen, Stefan Institute of Molecular Systems Biology, ETH Zürich, Zurich, Switzerland
- Keller, Markus A Cambridge Systems Biology Centre, University of Cambridge, Cambridge, United Kingdom
- Ralser, Markus ORCID Mill Hill Laboratory, The Francis Crick Institute, London, United Kingdom
- 2015-10-26
Published in:
- eLife. - eLife Sciences Publications, Ltd. - 2015, vol. 4
English
Metabolite exchange among co-growing cells is frequent by nature, however, is not necessarily occurring at growth-relevant quantities indicative of non-cell-autonomous metabolic function. Complementary auxotrophs of Saccharomyces cerevisiae amino acid and nucleotide metabolism regularly fail to compensate for each other's deficiencies upon co-culturing, a situation which implied the absence of growth-relevant metabolite exchange interactions. Contrastingly, we find that yeast colonies maintain a rich exometabolome and that cells prefer the uptake of extracellular metabolites over self-synthesis, indicators of ongoing metabolite exchange. We conceived a system that circumvents co-culturing and begins with a self-supporting cell that grows autonomously into a heterogeneous community, only able to survive by exchanging histidine, leucine, uracil, and methionine. Compensating for the progressive loss of prototrophy, self-establishing communities successfully obtained an auxotrophic composition in a nutrition-dependent manner, maintaining a wild-type like exometabolome, growth parameters, and cell viability. Yeast, as a eukaryotic model, thus possesses extensive capacity for growth-relevant metabolite exchange and readily cooperates in metabolism within progressively establishing communities.
- Language
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- English
- Open access status
- gold
- Identifiers
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- DOI 10.7554/elife.09943
- ISSN 2050-084X
- Persistent URL
- https://sonar.ch/global/documents/209246
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