Complete genome sequence of Dehalobacter restrictus PER-K23(T.).

Kruse T; Maillard J; Goodwin L; Woyke T; Teshima H; Bruce D; Detter C; Tapia R; Han C; Huntemann M; Wei CL; Han J; Chen A; Kyrpides N; Szeto E; Markowitz V; Ivanova N; Pagani I; Pati A; Pitluck S; Nolan M; Holliger C; Smidt H

doi:10.4056/sigs.3787426

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Complete genome sequence of Dehalobacter restrictus PER-K23(T.).

Kruse T Wageningen University, Agrotechnology and Food Sciences, Laboratory of Microbiology, Dreijenplein 10, NL-6703 HB Wageningen, The Netherlands.
Maillard J Ecole Polytechnique Fédérale de Lausanne (EPFL), School of Architecture, Civil and Environmental Engineering, Laboratory for Environmental Biotechnology, Station 6, CH-1015 Lausanne, Switzerland.
Goodwin L DOE Joint Genome Institute, Walnut Creek, California, USA ; Los Alamos National Laboratory, Bioscience Division, Los Alamos, New Mexico, USA.
Woyke T DOE Joint Genome Institute, Walnut Creek, California, USA.
Teshima H DOE Joint Genome Institute, Walnut Creek, California, USA ; Los Alamos National Laboratory, Bioscience Division, Los Alamos, New Mexico, USA.
Bruce D DOE Joint Genome Institute, Walnut Creek, California, USA ; Los Alamos National Laboratory, Bioscience Division, Los Alamos, New Mexico, USA.
Detter C DOE Joint Genome Institute, Walnut Creek, California, USA ; Los Alamos National Laboratory, Bioscience Division, Los Alamos, New Mexico, USA.
Tapia R DOE Joint Genome Institute, Walnut Creek, California, USA ; Los Alamos National Laboratory, Bioscience Division, Los Alamos, New Mexico, USA.
Han C DOE Joint Genome Institute, Walnut Creek, California, USA ; Los Alamos National Laboratory, Bioscience Division, Los Alamos, New Mexico, USA.
Huntemann M DOE Joint Genome Institute, Walnut Creek, California, USA.
Wei CL DOE Joint Genome Institute, Walnut Creek, California, USA.
Han J DOE Joint Genome Institute, Walnut Creek, California, USA.
Chen A DOE Joint Genome Institute, Walnut Creek, California, USA.
Kyrpides N DOE Joint Genome Institute, Walnut Creek, California, USA.
Szeto E DOE Joint Genome Institute, Walnut Creek, California, USA.
Markowitz V DOE Joint Genome Institute, Walnut Creek, California, USA.
Ivanova N DOE Joint Genome Institute, Walnut Creek, California, USA.
Pagani I DOE Joint Genome Institute, Walnut Creek, California, USA.
Pati A DOE Joint Genome Institute, Walnut Creek, California, USA.
Pitluck S DOE Joint Genome Institute, Walnut Creek, California, USA.
Nolan M DOE Joint Genome Institute, Walnut Creek, California, USA.
Holliger C Ecole Polytechnique Fédérale de Lausanne (EPFL), School of Architecture, Civil and Environmental Engineering, Laboratory for Environmental Biotechnology, Station 6, CH-1015 Lausanne, Switzerland.
Smidt H Wageningen University, Agrotechnology and Food Sciences, Laboratory of Microbiology, Dreijenplein 10, NL-6703 HB Wageningen, The Netherlands.

2014-02-07

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Standards in genomic sciences. - 2013

Dehalobacter restrictus type strain

English Dehalobacter restrictus strain PER-K23 (DSM 9455) is the type strain of the species Dehalobacter restrictus. D. restrictus strain PER-K23 grows by organohalide respiration, coupling the oxidation of H2 to the reductive dechlorination of tetra- or trichloroethene. Growth has not been observed with any other electron donor or acceptor, nor has fermentative growth been shown. Here we introduce the first full genome of a pure culture within the genus Dehalobacter. The 2,943,336 bp long genome contains 2,826 protein coding and 82 RNA genes, including 5 16S rRNA genes. Interestingly, the genome contains 25 predicted reductive dehalogenase genes, the majority of which appear to be full length. The reductive dehalogenase genes are mainly located in two clusters, suggesting a much larger potential for organohalide respiration than previously anticipated.

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