Journal article
Coherent manipulation of free amino acids fluorescence.
- Rondi A GAP-Biophotonics, University of Geneva, 22 ch. de Pinchat, CH-1211 Geneva 4, Switzerland.
- Bonacina L
- Trisorio A
- Hauri C
- Wolf JP
- 2012-03-08
Published in:
- Physical chemistry chemical physics : PCCP. - 2012
English
Coherent manipulation of molecular wavepackets in biomolecules might contribute to the quest towards label-free cellular imaging and protein identification. We report the use of optimally tailored UV laser pulses in pump-probe depletion experiments that selectively enhance or decrease fluorescence between two aromatic amino acids: tryptophan (Trp) and tyrosine (Tyr). Selective fluorescence modulation is achieved with a contrast of ~35%. A neat modification of the time-dependent fluorescence depletion signal of Trp is observed, while the Tyr transient trace remains unchanged. The mechanism invoked for explaining the change of the depletion of Trp is a less efficient coupling between the fluorescing state and the higher non-radiative excited states by the optimally shaped pulse, than by the reference pulse.
- Language
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- English
- Open access status
- closed
- Identifiers
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- DOI 10.1039/c2cp23357f
- PMID 22395710
- Persistent URL
- https://sonar.ch/global/documents/21352
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