Journal article

Potential in vivo transfer of a blaCTX-M14-harbouring plasmid established by combining long- and short-read sequencing.

  • Xavier BB Laboratory of Medical Microbiology, Vaccine & Infectious Disease Institute, Universiteit Antwerpen, Antwerp, Belgium.
  • Renzi G Clinical Microbiology Laboratory, University of Geneva Hospitals, Faculty of Medicine, Geneva, Switzerland.
  • Lammens C Laboratory of Medical Microbiology, Vaccine & Infectious Disease Institute, Universiteit Antwerpen, Antwerp, Belgium.
  • Cherkaoui A Clinical Microbiology Laboratory, University of Geneva Hospitals, Faculty of Medicine, Geneva, Switzerland.
  • Goossens H Laboratory of Medical Microbiology, Vaccine & Infectious Disease Institute, Universiteit Antwerpen, Antwerp, Belgium.
  • Schrenzel J Clinical Microbiology Laboratory, University of Geneva Hospitals, Faculty of Medicine, Geneva, Switzerland.
  • Harbarth S Infection Control Program, University of Geneva Hospitals, Faculty of Medicine, Geneva, Switzerland.
  • Malhotra-Kumar S Laboratory of Medical Microbiology, Vaccine & Infectious Disease Institute, Universiteit Antwerpen, Antwerp, Belgium. Electronic address: surbhi.malhotra@uantwerpen.be.
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  • 2019-02-11
Published in:
  • Journal of microbiological methods. - 2019
English Horizontal transfer of plasmid-mediated antibiotic resistance has rarely been documented in vivo. Utilizing long-read (Oxford Nanopore) and short-read (Illumina) sequencing, we confirmed that a gut-colonizing Escherichia coli and a hypervirulent Klebsiella pneumoniae ST23, isolated from a surgical site culture of a patient receiving cefuroxime therapy, harboured a 100% identical blaCTX-M-14-harbouring plasmid, indicative of a potential transfer in vivo.
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https://sonar.ch/global/documents/213812
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