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Journal article

Posttranscriptional trans-activation in cauliflower mosaic virus.

  • 1989-12-22
Published in:
  • Cell. - 1989
Base Sequence
Brassica
Genes
Genes, Viral
Molecular Sequence Data
Mosaic Viruses
Mutation
Plants
Plasmids
Protoplasts
RNA Processing, Post-Transcriptional
RNA, Messenger
Transcriptional Activation
English The ability of plant cells to translate dicistronic mRNAs that mimic a segment of the polycistronic 35S RNA from cauliflower mosaic virus has been tested. The chloramphenicol acetyltransferase and beta-glucuronidase open reading frames (ORFs) were fused in-frame to the second viral cistron (ORF I). Efficient reporter expression from the corresponding plasmids in plant protoplasts was observed only upon cotransfection with viral DNA. The trans-activating gene maps at ORF VI, which is expressed from a separate, monocistronic messenger (19S RNA). Deletion analysis shows that trans-activation selectively enhances downstream gene expression; the high expression of the upstream ORF is not further increased. The major reporter transcript remained bicistronic upon trans-activation, and its abundance varied only to a limited extent. Results indicate that trans-activation enhances the translation of downstream ORFs on polycistronic mRNAs derived from cauliflower mosaic virus.
Language
  • English
Open access status
bronze
Identifiers
Persistent URL
https://sonar.ch/global/documents/214242
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