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A functional Bucky ball-GFP transgene visualizes germ plasm in living zebrafish.
Journal article

A functional Bucky ball-GFP transgene visualizes germ plasm in living zebrafish.

  • Riemer S Institut für Entwicklungsbiochemie, GZMB, Universitätsmedizin Göttingen, Georg-August-Universität, Göttingen, Germany.
  • Bontems F Laboratory of Metabolism, Department of Internal Medicine Specialties, Faculty of Medicine, University of Geneva, Switzerland.
  • Krishnakumar P Institut für Entwicklungsbiochemie, GZMB, Universitätsmedizin Göttingen, Georg-August-Universität, Göttingen, Germany.
  • Gömann J Institut für Entwicklungsbiochemie, GZMB, Universitätsmedizin Göttingen, Georg-August-Universität, Göttingen, Germany.
  • Dosch R Institut für Entwicklungsbiochemie, GZMB, Universitätsmedizin Göttingen, Georg-August-Universität, Göttingen, Germany. Electronic address: roland.dosch@med.uni-goettingen.de.
  • 2015-07-06
Published in:
  • Gene expression patterns : GEP. - 2015
Balbiani body
Bucky ball
Germline development
Primordial germ cell
Transgene
Vasa
Animals
Animals, Genetically Modified
Antibodies
Germ Cells
Green Fluorescent Proteins
Larva
Oocytes
Oogenesis
Recombinant Fusion Proteins
Zebrafish
Zebrafish Proteins
English In many animals, the germline is specified by maternal RNA-granules termed germ plasm. The correct localization of germ plasm during embryogenesis is therefore crucial for the specification of germ cells. In zebrafish, we previously identified Bucky ball (Buc) as a key regulator of germ plasm formation. Here, we used a Buc antibody to describe its continuous germ plasm localization. Moreover, we generated a transgenic Buc-GFP line for live imaging, which visualizes germ plasm from its assembly during oogenesis up to the larval stages. Live imaging of Buc-GFP generated stunning movies, as they highlighted the dynamic details of germ plasm movements. Moreover, we discovered that Buc was still detected in primordial germ cells 2 days after fertilization. Interestingly, the transgene rescued buc mutants demonstrating genetically that the Buc-GFP fusion protein is functional. These results show that Buc-GFP exerts all biochemical interactions essential for germline development and highlight the potential of this line to analyze the molecular regulation of germ plasm formation.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/21514
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