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Is BAC Transgenesis Obsolete? State of the Art in the Era of Designer Nucleases

  • Beil, J. ORCID Institute for Medical Microbiology, Immunology and Hygiene, Technische Universität München, Trogerstraße 30, 81679 Munich, Germany
  • Fairbairn, L. Institute for Medical Microbiology, Immunology and Hygiene, Technische Universität München, Trogerstraße 30, 81679 Munich, Germany
  • Pelczar, P. Institute of Animal Laboratory Sciences, VetSuisse Faculty, University of Zurich, Winterthurer Straße 190, 8057 Zurich, Switzerland
  • Buch, T. ORCID Institute for Medical Microbiology, Immunology and Hygiene, Technische Universität München, Trogerstraße 30, 81679 Munich, Germany
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  • Journal of Biomedicine and Biotechnology. - Hindawi Limited. - 2012, vol. 2012, p. 1-5
English DNA constructs based on bacterial artificial chromosomes (BACs) are frequently used to generate transgenic animals as they reduce the influence of position effects and allow predictable expression patterns for genes whose regulatory sequences are not fully identified. Despite these advantages BAC transgenics suffer from drawbacks such as complicated vector construction, low efficiency of transgenesis, and some remaining expression variegation. The recent development of transcription activator-like effector nucleases (TALENs) and zinc finger nucleases (ZFNs) has resulted in new transgenic techniques which do not have the drawbacks associated with BAC transgenesis. Initial reports indicate that such designer nucleases (DNs) allow the targeted insertion of transgenes into endogenous loci by direct injection of the targeting vector and mRNA/DNA encoding the predesigned nucleases into oocytes. This results in the transgene being inserted at a specific locus in the mouse genome, thus circumventing the drawbacks associated with BAC transgenesis.
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  • English
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gold
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https://sonar.ch/global/documents/215416
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