Back
Full-text
Journal article

COP1 is required for UV-B-induced nuclear accumulation of the UVR8 photoreceptor.

  • Yin R Department of Botany and Plant Biology, University of Geneva, CH-1211 Geneva 4, Switzerland;
  • Skvortsova MY Department of Botany and Plant Biology, University of Geneva, CH-1211 Geneva 4, Switzerland;
  • Loubéry S Department of Botany and Plant Biology, University of Geneva, CH-1211 Geneva 4, Switzerland;
  • Ulm R Department of Botany and Plant Biology, University of Geneva, CH-1211 Geneva 4, Switzerland; Institute of Genetics and Genomics of Geneva, University of Geneva, CH-1211 Geneva 4, Switzerland roman.ulm@unige.ch.
  • 2016-07-14
Published in:
  • Proceedings of the National Academy of Sciences of the United States of America. - 2016
COP1
UV-B photoreceptor
UVR8
glucocorticoid receptor
nuclear accumulation
Active Transport, Cell Nucleus
Arabidopsis
Arabidopsis Proteins
Cell Nucleus
Chromosomal Proteins, Non-Histone
Gene Expression Regulation, Plant
Immunoblotting
Mutation
Photoreceptors, Plant
Plants, Genetically Modified
Signal Transduction
Ubiquitin-Protein Ligases
Ultraviolet Rays
English The UV-B photoreceptor UV RESISTANCE LOCUS 8 (UVR8) promotes UV-B acclimation and tolerance in Arabidopsis thaliana UVR8 localizes to both cytosol and nucleus, but its main activity is assumed to be nuclear. UV-B photoreception stimulates nuclear accumulation of UVR8 in a presently unknown manner. Here, we show that CONSTITUTIVELY PHOTOMORPHOGENIC 1 (COP1) is required for UV-B-induced nuclear accumulation of UVR8, but bypassing the COP1 requirement for UVR8 nuclear accumulation did not rescue the cop1 mutant UV-B phenotype. Using a glucocorticoid receptor (GR)-based fusion protein system to conditionally localize GR-UVR8 to the nucleus, we have demonstrated that both photoactivation and nuclear localization of UVR8 are required for UV-B-induced photomorphogenic responses. In contrast, there was no UV-B response when UV-B-activated UVR8 was artificially retained in the cytosol. In agreement with a predominantly nuclear activity, constitutively active UVR8(W285A) accumulated in the nucleus also in the absence of UV-B. Furthermore, GR-COP1 expression lines suggested that UV-B-activated UVR8 can be coimported into the nucleus by COP1. Our data strongly support localization of UVR8 signaling in the nucleus and a dual role for COP1 in the regulation of UV-B-induced UVR8 nuclear accumulation and in UVR8-mediated UV-B signaling.
Language
  • English
Open access status
bronze
Identifiers
Persistent URL
https://sonar.ch/global/documents/216138
Statistics
Document views: 47 File downloads:
  • Full-text: 0