A method for the study of fungal growth inhibition by plant proteins.
- 1990-05-01
Published in:
- FEMS microbiology letters. - 1990
Fungi
Hydrogen-Ion Concentration
Microbial Sensitivity Tests
Plant Proteins
Spectrum Analysis
Spores, Fungal
Temperature
English
A bioassay is described for the study of inhibitory activity of plant proteins on fungal growth. Fungal spores were germinated in liquid growth medium and pipetted into wells of a microtitre plate. Fungal growth was followed spectrophotometrically. The bioassay was tested using crude protein extracts from plant tissues known to have high activities of chitinase and beta-1,3-glucanase, and with purified enzymes. Crude protein preparations and combinations of the purified enzymes produced a temporary reduction of growth but no permanent growth inhibition.
- Language
-
- English
- Open access status
- bronze
- Identifiers
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- DOI 10.1016/0378-1097(90)90413-k
- PMID 2379812
- Persistent URL
- https://sonar.ch/global/documents/216663
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