Metal-responsive transcription factor (MTF-1) handles both extremes, copper load and copper starvation, by activating different genes.
- Selvaraj A Institute of Molecular Biology, University of Zurich, CH-8057, Zurich, Switzerland.
- Balamurugan K
- Yepiskoposyan H
- Zhou H
- Egli D
- Georgiev O
- Thiele DJ
- Schaffner W
- 2005-04-19
Published in:
- Genes & development. - 2005
Animals
Biological Transport
Cation Transport Proteins
Cells, Cultured
Computational Biology
Copper
Copper Transporter 1
DNA-Binding Proteins
Drosophila
Electrophoretic Mobility Shift Assay
Gene Components
Gene Expression Regulation
Green Fluorescent Proteins
Ion Transport
Metallothionein
Protein Binding
Transcription Factors
Transfection
English
From insects to mammals, metallothionein genes are induced in response to heavy metal load by the transcription factor MTF-1, which binds to short DNA sequence motifs, termed metal response elements (MREs). Here we describe a novel and seemingly paradoxical role for MTF-1 in Drosophila in that it also mediates transcriptional activation of Ctr1B, a copper importer, upon copper depletion. Activation depends on the same type of MRE motifs in the upstream region of the Ctr1B gene as are normally required for metal induction. Thus, a single transcription factor, MTF-1, plays a direct role in both copper detoxification and acquisition by inducing the expression of metallothioneins and of a copper importer, respectively.
- Language
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- English
- Open access status
- gold
- Identifiers
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- DOI 10.1101/gad.1301805
- PMID 15833915
- Persistent URL
- https://sonar.ch/global/documents/219272
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