Journal article
Exploring variation in proanthocyanidin composition and content of sainfoin (Onobrychis viciifolia).
- Azuhnwi BN Agroscope Liebefeld-Posieux Research Station ALP, 1725 Posieux, Switzerland.
- Boller B
- Dohme-Meier F
- Hess HD
- Kreuzer M
- Stringano E
- Mueller-Harvey I
- 2013-03-02
Published in:
- Journal of the science of food and agriculture. - 2013
Animal Feed
Animals
Anthelmintics
Chromatography, Gel
Chromatography, High Pressure Liquid
Crops, Agricultural
Fabaceae
Hydrolysis
Hydroxylation
Molecular Structure
Molecular Weight
Nutritive Value
Plant Components, Aerial
Proanthocyanidins
Seasons
Species Specificity
Stereoisomerism
Switzerland
English
BACKGROUND
To maximise the potential benefits to ruminants from sainfoin, plant breeding should focus on developing varieties with predictable condensed tannin (CT) profiles. Little is known about whether and to what extent accession and environment influence sainfoin CT structures. We sought to investigate the likely extent of accession and environment effects on CT characteristics of sainfoin. Four single-flowering (Communis) accessions and two multiple-flowering (Bifera) accessions, grown at three sites and collected at two harvest times were used. Sainfoin CTs were characterised by thiolytic degradation and by high-performance liquid chromatography-gel permeation chromatography (HPLC-GPC). Also, CT concentration measured earlier by the HCl-butanol method was compared with that from thiolysis.
RESULTS
Thiolysis revealed that accession and harvest influenced most CT structural attributes. Bifera CTs eluted as single peaks (Mp < 6220 Da) in HPLC-GPC across the two harvests and two sites, whereas Communis generated two to three CT peaks, which included a peak (Mp ≤ 9066 Da) in the second harvest. A discrepancy was observed in CT concentrations measured by the two methods.
CONCLUSION
CTs from Bifera accessions had more stable and predictable characteristics across harvests and sites and this could be of interest when breeding sainfoin.
To maximise the potential benefits to ruminants from sainfoin, plant breeding should focus on developing varieties with predictable condensed tannin (CT) profiles. Little is known about whether and to what extent accession and environment influence sainfoin CT structures. We sought to investigate the likely extent of accession and environment effects on CT characteristics of sainfoin. Four single-flowering (Communis) accessions and two multiple-flowering (Bifera) accessions, grown at three sites and collected at two harvest times were used. Sainfoin CTs were characterised by thiolytic degradation and by high-performance liquid chromatography-gel permeation chromatography (HPLC-GPC). Also, CT concentration measured earlier by the HCl-butanol method was compared with that from thiolysis.
RESULTS
Thiolysis revealed that accession and harvest influenced most CT structural attributes. Bifera CTs eluted as single peaks (Mp < 6220 Da) in HPLC-GPC across the two harvests and two sites, whereas Communis generated two to three CT peaks, which included a peak (Mp ≤ 9066 Da) in the second harvest. A discrepancy was observed in CT concentrations measured by the two methods.
CONCLUSION
CTs from Bifera accessions had more stable and predictable characteristics across harvests and sites and this could be of interest when breeding sainfoin.
- Language
-
- English
- Open access status
- closed
- Identifiers
-
- DOI 10.1002/jsfa.6119
- PMID 23450773
- Persistent URL
- https://sonar.ch/global/documents/220044
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