A hybrid sub-lineage of Listeria monocytogenes comprising hypervirulent isolates.

Yin Y; Yao H; Doijad S; Kong S; Shen Y; Cai X; Tan W; Wang Y; Feng Y; Ling Z; Wang G; Hu Y; Lian K; Sun X; Liu Y; Wang C; Jiao K; Liu G; Song R; Chen X; Pan Z; Loessner MJ; Chakraborty T; Jiao X

doi:10.1038/s41467-019-12072-1

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Journal article

A hybrid sub-lineage of Listeria monocytogenes comprising hypervirulent isolates.

Yin Y Jiangsu Key Laboratory of Zoonosis, Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, Ministry of Agriculture and Rural Affairs of the People's Republic of China, Yangzhou University, 48 East Wenhui Road, Yangzhou, 225009, Jiangsu Province, China. yylan@yzu.edu.cn.
Yao H Jiangsu Key Laboratory of Zoonosis, Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, Ministry of Agriculture and Rural Affairs of the People's Republic of China, Yangzhou University, 48 East Wenhui Road, Yangzhou, 225009, Jiangsu Province, China.
Doijad S Institute of Medical Microbiology, Justus-Liebig University, Giessen, 35394, Germany.
Kong S Jiangsu Key Laboratory of Zoonosis, Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, Ministry of Agriculture and Rural Affairs of the People's Republic of China, Yangzhou University, 48 East Wenhui Road, Yangzhou, 225009, Jiangsu Province, China.
Shen Y Laboratory of Food Microbiology, Institute of Food, Nutrition and Health, ETH Zurich, 8092, Zurich, Switzerland.
Cai X Jiangsu Key Laboratory of Zoonosis, Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, Ministry of Agriculture and Rural Affairs of the People's Republic of China, Yangzhou University, 48 East Wenhui Road, Yangzhou, 225009, Jiangsu Province, China.
Tan W Jiangsu Key Laboratory of Zoonosis, Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, Ministry of Agriculture and Rural Affairs of the People's Republic of China, Yangzhou University, 48 East Wenhui Road, Yangzhou, 225009, Jiangsu Province, China.
Wang Y Jiangsu Key Laboratory of Zoonosis, Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, Ministry of Agriculture and Rural Affairs of the People's Republic of China, Yangzhou University, 48 East Wenhui Road, Yangzhou, 225009, Jiangsu Province, China.
Feng Y Jiangsu Key Laboratory of Zoonosis, Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, Ministry of Agriculture and Rural Affairs of the People's Republic of China, Yangzhou University, 48 East Wenhui Road, Yangzhou, 225009, Jiangsu Province, China.
Ling Z Jiangsu Key Laboratory of Zoonosis, Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, Ministry of Agriculture and Rural Affairs of the People's Republic of China, Yangzhou University, 48 East Wenhui Road, Yangzhou, 225009, Jiangsu Province, China.
Wang G Jiangsu Key Laboratory of Zoonosis, Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, Ministry of Agriculture and Rural Affairs of the People's Republic of China, Yangzhou University, 48 East Wenhui Road, Yangzhou, 225009, Jiangsu Province, China.
Hu Y Jiangsu Key Laboratory of Zoonosis, Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, Ministry of Agriculture and Rural Affairs of the People's Republic of China, Yangzhou University, 48 East Wenhui Road, Yangzhou, 225009, Jiangsu Province, China.
Lian K Jiangsu Key Laboratory of Zoonosis, Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, Ministry of Agriculture and Rural Affairs of the People's Republic of China, Yangzhou University, 48 East Wenhui Road, Yangzhou, 225009, Jiangsu Province, China.
Sun X Jiangsu Key Laboratory of Zoonosis, Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, Ministry of Agriculture and Rural Affairs of the People's Republic of China, Yangzhou University, 48 East Wenhui Road, Yangzhou, 225009, Jiangsu Province, China.
Liu Y China Animal Disease Control Center, No.17 Tiangui Street, Daxing District, 102618, Beijing, China.
Wang C China Animal Disease Control Center, No.17 Tiangui Street, Daxing District, 102618, Beijing, China.
Jiao K Jiangsu Key Laboratory of Zoonosis, Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, Ministry of Agriculture and Rural Affairs of the People's Republic of China, Yangzhou University, 48 East Wenhui Road, Yangzhou, 225009, Jiangsu Province, China.
Liu G Xuyi Center for Animal Disease Control and Prevention, Xuyi City, Jiangsu Province, China.
Song R Jiangsu Key Laboratory of Zoonosis, Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, Ministry of Agriculture and Rural Affairs of the People's Republic of China, Yangzhou University, 48 East Wenhui Road, Yangzhou, 225009, Jiangsu Province, China.
Chen X Jiangsu Key Laboratory of Zoonosis, Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, Ministry of Agriculture and Rural Affairs of the People's Republic of China, Yangzhou University, 48 East Wenhui Road, Yangzhou, 225009, Jiangsu Province, China.
Pan Z Jiangsu Key Laboratory of Zoonosis, Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, Ministry of Agriculture and Rural Affairs of the People's Republic of China, Yangzhou University, 48 East Wenhui Road, Yangzhou, 225009, Jiangsu Province, China.
Loessner MJ Laboratory of Food Microbiology, Institute of Food, Nutrition and Health, ETH Zurich, 8092, Zurich, Switzerland.
Chakraborty T Jiangsu Key Laboratory of Zoonosis, Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, Ministry of Agriculture and Rural Affairs of the People's Republic of China, Yangzhou University, 48 East Wenhui Road, Yangzhou, 225009, Jiangsu Province, China. Trinad.Chakraborty@mikrobio.med.uni-giessen.de.
Jiao X Jiangsu Key Laboratory of Zoonosis, Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, Ministry of Agriculture and Rural Affairs of the People's Republic of China, Yangzhou University, 48 East Wenhui Road, Yangzhou, 225009, Jiangsu Province, China. jiao@yzu.edu.cn.

2019-10-02

Published in:

Nature communications. - 2019

English The foodborne pathogen Listeria monocytogenes (Lm) is a highly heterogeneous species and currently comprises of 4 evolutionarily distinct lineages. Here, we characterize isolates from severe ovine listeriosis outbreaks that represent a hybrid sub-lineage of the major lineage II (HSL-II) and serotype 4h. HSL-II isolates are highly virulent and exhibit higher organ colonization capacities than well-characterized hypervirulent strains of Lm in an orogastric mouse infection model. The isolates harbour both the Lm Pathogenicity Island (LIPI)-1 and a truncated LIPI-2 locus, encoding sphingomyelinase (SmcL), a virulence factor required for invasion and bacterial translocation from the gut, and other non-contiguous chromosomal segments from another pathogenic species, L. ivanovii. HSL-II isolates exhibit a unique wall teichoic acid (WTA) structure essential for resistance to antimicrobial peptides, bacterial invasion and virulence. The discovery of isolates harbouring pan-species virulence genes of the genus Listeria warrants global efforts to identify further hypervirulent lineages of Lm.

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