Fluorescence-Based Binding Assay for Screening Ligands of Angiotensin Receptors.

Bragina ME; Stergiopulos N; Fraga-Silva RA

doi:10.1007/978-1-4939-7030-8_13

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Journal article

Fluorescence-Based Binding Assay for Screening Ligands of Angiotensin Receptors.

Bragina ME Laboratory of Hemodynamics and Cardiovascular Technology, Institute of Bioengineering, Ecole Polytechnique Fédérale de Lausanne, Station 17, BM 5115, 1015, Lausanne, Switzerland.
Stergiopulos N Laboratory of Hemodynamics and Cardiovascular Technology, Institute of Bioengineering, Ecole Polytechnique Fédérale de Lausanne, Station 17, BM 5115, 1015, Lausanne, Switzerland.
Fraga-Silva RA Laboratory of Hemodynamics and Cardiovascular Technology, Institute of Bioengineering, Ecole Polytechnique Fédérale de Lausanne, Station 17, BM 5115, 1015, Lausanne, Switzerland.

2017-05-14

Published in:

Methods in molecular biology (Clifton, N.J.). - 2017

English Binding assay is a common technique used to characterize ability of a ligand to interact with a specific biological target. A number of parameters, such as binding affinity, receptor density, and association/dissociation rate constants, can be measured by means of this technique. In most cases, implementation of the binding assay requires specific infrastructure for labeling and detecting the ligand, which impedes realization of this technique in a standard laboratory. Here we describe a simple fluorescence-based binding assay for angiotensin peptides and receptors, which does not require complex equipment and can be used for initial screening of the novel ligands or mutational studies.

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