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Cloning and functional analysis of alkB genes in Alcanivorax borkumensis SK2.
Journal article

Cloning and functional analysis of alkB genes in Alcanivorax borkumensis SK2.

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  • 2004-02-12
Published in:
  • Environmental microbiology. - 2004
Alkanes
Bacterial Proteins
Biodegradation, Environmental
Cloning, Molecular
Cluster Analysis
Cytochrome P-450 CYP4A
DNA, Bacterial
Genes, Bacterial
Halomonadaceae
Hexanes
Molecular Sequence Data
Mutagenesis, Insertional
Octanes
Phylogeny
Sequence Analysis, DNA
English Alcanivorax is an alkane-degrading marine bacterium which propagates and becomes predominant in crude-oil-containing seawater when nitrogen and phosphorus nutrients are supplemented. To identify the genes responsible for alkane degradation in this organism, two putative genes for alkane hydroxylases were cloned from Alcanivorax borkumensis SK2. They were named alkB1 and alkB2. These genes were subsequently disrupted in A. borkumensis SK2, and the growth phenotypes of the disruptants were examined. The results indicate that the alkB1 gene is responsible for the degradation of short-chain n-alkanes. A double mutant defective in both alkB1 and alkB2 was still able to grow on medium-chain n-alkanes, indicating that genes other than alkB1 and alkB2 are also involved in n-alkane hydroxylation by A. borkumensis SK2.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/222210
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