Journal article
Plasmodium Condensin Core Subunits SMC2/SMC4 Mediate Atypical Mitosis and Are Essential for Parasite Proliferation and Transmission.
- Pandey R School of Life Sciences, Queens Medical Centre, University of Nottingham, Nottingham NG7 2UH, UK.
- Abel S Department of Molecular, Cell and Systems Biology, University of California Riverside, 900 University Ave., Riverside, CA 92521, USA.
- Boucher M School of Life Sciences, Queens Medical Centre, University of Nottingham, Nottingham NG7 2UH, UK.
- Wall RJ Wellcome Trust Centre for Anti-Infectives Research, School of Life Sciences, University of Dundee, Dundee DD1 5EH, UK.
- Zeeshan M School of Life Sciences, Queens Medical Centre, University of Nottingham, Nottingham NG7 2UH, UK.
- Rea E School of Life Sciences, Queens Medical Centre, University of Nottingham, Nottingham NG7 2UH, UK.
- Freville A School of Life Sciences, Queens Medical Centre, University of Nottingham, Nottingham NG7 2UH, UK.
- Lu XM Department of Molecular, Cell and Systems Biology, University of California Riverside, 900 University Ave., Riverside, CA 92521, USA.
- Brady D School of Life Sciences, Queens Medical Centre, University of Nottingham, Nottingham NG7 2UH, UK.
- Daniel E School of Life Sciences, Queens Medical Centre, University of Nottingham, Nottingham NG7 2UH, UK.
- Stanway RR Institute of Cell Biology, University of Bern, Bern 3012, Switzerland.
- Wheatley S School of Life Sciences, Queens Medical Centre, University of Nottingham, Nottingham NG7 2UH, UK.
- Batugedara G Department of Molecular, Cell and Systems Biology, University of California Riverside, 900 University Ave., Riverside, CA 92521, USA.
- Hollin T Department of Molecular, Cell and Systems Biology, University of California Riverside, 900 University Ave., Riverside, CA 92521, USA.
- Bottrill AR School of Life Sciences, Gibbet Hill Campus, University of Warwick, Coventry CV4 7AL, UK.
- Gupta D Translational Bioinformatics Group, International Center for Genetic Engineering and Biotechnology, New Delhi 110067, India.
- Holder AA Malaria Parasitology Laboratory, The Francis Crick Institute, London NW1 1AT, UK.
- Le Roch KG Department of Molecular, Cell and Systems Biology, University of California Riverside, 900 University Ave., Riverside, CA 92521, USA. Electronic address: karine.leroch@ucr.edu.
- Tewari R School of Life Sciences, Queens Medical Centre, University of Nottingham, Nottingham NG7 2UH, UK. Electronic address: rita.tewari@nottingham.ac.uk.
- 2020-02-13
Published in:
- Cell reports. - 2020
Plasmodium
SMC4
atypical mitosis
chromosome
condensin
kleisin
mosquito
oocyst
protein complex
transmision
English
Condensin is a multi-subunit protein complex regulating chromosome condensation and segregation during cell division. In Plasmodium spp., the causative agent of malaria, cell division is atypical and the role of condensin is unclear. Here we examine the role of SMC2 and SMC4, the core subunits of condensin, during endomitosis in schizogony and endoreduplication in male gametogenesis. During early schizogony, SMC2/SMC4 localize to a distinct focus, identified as the centromeres by NDC80 fluorescence and chromatin immunoprecipitation sequencing (ChIP-seq) analyses, but do not form condensin I or II complexes. In mature schizonts and during male gametogenesis, there is a diffuse SMC2/SMC4 distribution on chromosomes and in the nucleus, and both condensin I and condensin II complexes form at these stages. Knockdown of smc2 and smc4 gene expression reveals essential roles in parasite proliferation and transmission. The condensin core subunits (SMC2/SMC4) form different complexes and may have distinct functions at various stages of the parasite life cycle.
- Language
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- English
- Open access status
- gold
- Identifiers
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- DOI 10.1016/j.celrep.2020.01.033
- PMID 32049018
- Persistent URL
- https://sonar.ch/global/documents/222785
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