Multiprotein expression strategy for structural biology of eukaryotic complexes.
- Fitzgerald DJ ETH Zürich, Institut für Molekularbiologie und Biophysik, ETH-Hönggerberg, CH-8093 Zürich, Switzerland.
- Schaffitzel C
- Berger P
- Wellinger R
- Bieniossek C
- Richmond TJ
- Berger I
- 2007-03-16
Published in:
- Structure (London, England : 1993). - 2007
Animals
Baculoviridae
Cell Line
Escherichia coli
Genetic Vectors
Humans
Multiprotein Complexes
Spodoptera
English
The concept of the cell as a collection of multisubunit protein machines is emerging as a cornerstone of modern biology, and molecular-level study of these machines in most cases will require recombinant production. Here, we present and validate a strategy to rapidly produce, permutate, and posttranslationally modify large, eukaryotic multiprotein complexes by using DNA recombination in a process that is fully automatable. Parallel production of 12 protein complex variants within a period of weeks resulted in specimens of sufficient quantity and homogeneity for structural biology applications.
- Language
-
- English
- Open access status
- green
- Identifiers
-
- DOI 10.1016/j.str.2007.01.016
- PMID 17355863
- Persistent URL
- https://sonar.ch/global/documents/222871
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