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Highly multiplexed imaging of tumor tissues with subcellular resolution by mass cytometry.
Journal article

Highly multiplexed imaging of tumor tissues with subcellular resolution by mass cytometry.

  • Giesen C 1] Institute of Molecular Life Sciences, University of Zürich, Zürich, Switzerland. [2].
  • Wang HA 1] Department of Chemistry, Swiss Federal Institute of Technology Zürich, Zürich, Switzerland. [2] Swiss Light Source, Paul Scherrer Institute, Villigen, Switzerland. [3].
  • Schapiro D 1] Institute of Molecular Life Sciences, University of Zürich, Zürich, Switzerland. [2] Systems Biology Ph.D. Program, Life Science Zürich Graduate School, ETH Zürich and University of Zürich, Zürich, Switzerland.
  • Zivanovic N 1] Institute of Molecular Life Sciences, University of Zürich, Zürich, Switzerland. [2] Molecular Life Science Ph.D. Program, Life Science Zürich Graduate School, ETH Zürich and University of Zürich, Zürich, Switzerland.
  • Jacobs A Institute of Molecular Life Sciences, University of Zürich, Zürich, Switzerland.
  • Hattendorf B Department of Chemistry, Swiss Federal Institute of Technology Zürich, Zürich, Switzerland.
  • Schüffler PJ Department of Computer Science, Swiss Federal Institute of Technology Zürich, Zürich, Switzerland.
  • Grolimund D Swiss Light Source, Paul Scherrer Institute, Villigen, Switzerland.
  • Buhmann JM Department of Computer Science, Swiss Federal Institute of Technology Zürich, Zürich, Switzerland.
  • Brandt S Institute of Surgical Pathology, University Hospital Zürich, Zürich, Switzerland.
  • Varga Z Institute of Surgical Pathology, University Hospital Zürich, Zürich, Switzerland.
  • Wild PJ Institute of Surgical Pathology, University Hospital Zürich, Zürich, Switzerland.
  • Günther D Department of Chemistry, Swiss Federal Institute of Technology Zürich, Zürich, Switzerland.
  • Bodenmiller B Institute of Molecular Life Sciences, University of Zürich, Zürich, Switzerland.
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  • 2014-03-04
Published in:
  • Nature methods. - 2014
Breast Neoplasms
Cell Line
Epithelial Cells
Female
Gene Expression Regulation, Neoplastic
Humans
Image Cytometry
Neoplasm Proteins
English Mass cytometry enables high-dimensional, single-cell analysis of cell type and state. In mass cytometry, rare earth metals are used as reporters on antibodies. Analysis of metal abundances using the mass cytometer allows determination of marker expression in individual cells. Mass cytometry has previously been applied only to cell suspensions. To gain spatial information, we have coupled immunohistochemical and immunocytochemical methods with high-resolution laser ablation to CyTOF mass cytometry. This approach enables the simultaneous imaging of 32 proteins and protein modifications at subcellular resolution; with the availability of additional isotopes, measurement of over 100 markers will be possible. We applied imaging mass cytometry to human breast cancer samples, allowing delineation of cell subpopulations and cell-cell interactions and highlighting tumor heterogeneity. Imaging mass cytometry complements existing imaging approaches. It will enable basic studies of tissue heterogeneity and function and support the transition of medicine toward individualized molecularly targeted diagnosis and therapies.
Language
  • English
Open access status
closed
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Persistent URL
https://sonar.ch/global/documents/223237
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