Back
A nucleoside transporter from Trypanosoma brucei involved in drug resistance.
Journal article

A nucleoside transporter from Trypanosoma brucei involved in drug resistance.

  • 1999-07-10
Published in:
  • Science (New York, N.Y.). - 1999
Adenosine
Amino Acid Sequence
Animals
Arsenicals
Biological Transport
Carrier Proteins
Cloning, Molecular
Drug Resistance
Genes, Protozoan
Membrane Proteins
Molecular Sequence Data
Mutation
Nucleoside Transport Proteins
Nucleosides
Purines
Saccharomyces cerevisiae
Substrate Specificity
Trypanocidal Agents
Trypanosoma brucei brucei
Trypanosomiasis, African
English Drug resistance of pathogens is an increasing problem whose underlying mechanisms are not fully understood. Cellular uptake of the major drugs against Trypanosoma brucei spp., the causative agents of sleeping sickness, is thought to occur through an unusual, so far unidentified adenosine transporter. Saccharomyces cerevisiae was used in a functional screen to clone a gene (TbAT1) from Trypanosoma brucei brucei that encodes a nucleoside transporter. When expressed in yeast, TbAT1 enabled adenosine uptake and conferred susceptibility to melaminophenyl arsenicals. Drug-resistant trypanosomes harbor a defective TbAT1 variant. The molecular identification of the entry route of trypanocides opens the way to approaches for diagnosis and treatment of drug-resistant sleeping sickness.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/224063
Statistics
Document views: 20 File downloads: