Measuring in vivo protein half-life.
Journal article

Measuring in vivo protein half-life.

Show more…
  • 2011-06-25
Published in:
  • Chemistry & biology. - 2011
English Protein turnover critically influences many biological functions, yet methods have been lacking to assess this parameter in vivo. Here, we demonstrate how chemical labeling of SNAP-tag fusion proteins can be exploited to measure the half-life of resident intracellular and extracellular proteins in living mice. First, we demonstrate that SNAP-tag substrates have wide bioavailability in mice and can be used for the specific in vivo labeling of SNAP-tag fusion proteins. We then apply near-infrared probes to perform noninvasive imaging of in vivo-labeled tumors. Finally, we use SNAP-mediated chemical pulse-chase labeling to perform measurement of the in vivo half-life of different extra- and intracellular proteins. These results open broad perspectives for studying protein function in living animals.
Language
  • English
Open access status
gold
Identifiers
Persistent URL
https://sonar.ch/global/documents/225538
Statistics

Document views: 9 File downloads: