In vivo 13C MRS in the mouse brain at 14.1 Tesla and metabolic flux quantification under infusion of [1,6-13C2]glucose.
- Lai M 1 Laboratory for Functional and Metabolic Imaging (LIFMET), École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.
- Lanz B 1 Laboratory for Functional and Metabolic Imaging (LIFMET), École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.
- Poitry-Yamate C 2 Center for Biomedical Imaging, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.
- Romero JF 2 Center for Biomedical Imaging, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.
- Berset CM 2 Center for Biomedical Imaging, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.
- Cudalbu C 2 Center for Biomedical Imaging, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.
- Gruetter R 1 Laboratory for Functional and Metabolic Imaging (LIFMET), École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.
- 2017-10-20
Published in:
- Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism. - 2018
13C MRS
glucose metabolism
metabolic modelling
mouse brain
Animals
Brain
Carbon Isotopes
Glucose
Glutamic Acid
Glutamine
Magnetic Resonance Spectroscopy
Male
Mice
Models, Neurological
Neuroglia
Neurons
English
In vivo 13C magnetic resonance spectroscopy (MRS) enables the investigation of cerebral metabolic compartmentation while, e.g. infusing 13C-labeled glucose. Metabolic flux analysis of 13C turnover previously yielded quantitative information of glutamate and glutamine metabolism in humans and rats, while the application to in vivo mouse brain remains exceedingly challenging. In the present study, 13C direct detection at 14.1 T provided highly resolved in vivo spectra of the mouse brain while infusing [1,6-13C2]glucose for up to 5 h. 13C incorporation to glutamate and glutamine C4, C3, and C2 and aspartate C3 were detected dynamically and fitted to a two-compartment model: flux estimation of neuron-glial metabolism included tricarboxylic acid cycle (TCA) flux in astrocytes (Vg = 0.16 ± 0.03 µmol/g/min) and neurons (VTCAn = 0.56 ± 0.03 µmol/g/min), pyruvate carboxylase activity (VPC = 0.041 ± 0.003 µmol/g/min) and neurotransmission rate (VNT = 0.084 ± 0.008 µmol/g/min), resulting in a cerebral metabolic rate of glucose (CMRglc) of 0.38 ± 0.02 µmol/g/min, in excellent agreement with that determined with concomitant 18F-fluorodeoxyglucose positron emission tomography (18FDG PET).We conclude that modeling of neuron-glial metabolism in vivo is accessible in the mouse brain from 13C direct detection with an unprecedented spatial resolution under [1,6-13C2]glucose infusion.
- Language
-
- English
- Open access status
- bronze
- Identifiers
-
- DOI 10.1177/0271678X17734101
- PMID 29047296
- Persistent URL
- https://sonar.ch/global/documents/225883
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