Automated Platform for Long-Term Culture and High-Content Phenotyping of Single C. elegans Worms.
- Atakan HB Laboratory of Microsystems, Ecole Polytechnique Fédérale de Lausanne, CH-1015, Lausanne, Switzerland.
- Xiang R Laboratory of Microsystems, Ecole Polytechnique Fédérale de Lausanne, CH-1015, Lausanne, Switzerland.
- Cornaglia M Laboratory of Microsystems, Ecole Polytechnique Fédérale de Lausanne, CH-1015, Lausanne, Switzerland.
- Mouchiroud L Laboratory of Integrative Systems Physiology, Ecole Polytechnique Fédérale de Lausanne, CH-1015, Lausanne, Switzerland.
- Katsyuba E Laboratory of Integrative Systems Physiology, Ecole Polytechnique Fédérale de Lausanne, CH-1015, Lausanne, Switzerland.
- Auwerx J Laboratory of Integrative Systems Physiology, Ecole Polytechnique Fédérale de Lausanne, CH-1015, Lausanne, Switzerland.
- Gijs MAM Laboratory of Microsystems, Ecole Polytechnique Fédérale de Lausanne, CH-1015, Lausanne, Switzerland. martin.gijs@epfl.ch.
- 2019-10-06
Published in:
- Scientific reports. - 2019
Animals
Caenorhabditis elegans
Drug Evaluation, Preclinical
Embryonic Development
High-Throughput Screening Assays
Lab-On-A-Chip Devices
Larva
Microfluidic Analytical Techniques
Models, Animal
Phenotype
English
The nematode Caenorhabditis elegans is a suitable model organism in drug screening. Traditionally worms are grown on agar plates, posing many challenges for long-term culture and phenotyping of animals under identical conditions. Microfluidics allows for 'personalized' phenotyping, as microfluidic chips permit collecting individual responses over worms' full life. Here, we present a multiplexed, high-throughput, high-resolution microfluidic approach to culture C. elegans from embryo to the adult stage at single animal resolution. We allocated single embryos to growth chambers, for observing the main embryonic and post-embryonic development stages and phenotypes, while exposing worms to up to 8 different well-controlled chemical conditions. Our approach allowed eliminating bacteria aggregation and biofilm formation-related clogging issues, which enabled us performing up to 80 hours of automated single worm culture studies. Our microfluidic platform is linked with an automated phenotyping code that registers organism-associated phenotypes at high-throughput. We validated our platform with a dose-response study of the anthelmintic drug tetramisole by studying its influence through the life cycle of the nematodes. In parallel, we could observe development effects and variations in single embryo and worm viability due to the bleaching procedure that is standardly used for harvesting the embryos from a worm culture agar plate.
- Language
-
- English
- Open access status
- gold
- Identifiers
-
- DOI 10.1038/s41598-019-50920-8
- PMID 31586133
- Persistent URL
- https://sonar.ch/global/documents/226050
Statistics
Document views: 6
File downloads:
- fulltext.pdf: 0