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Dynamic Radiolabeling of S-Palmitoylated Proteins.
Journal article

Dynamic Radiolabeling of S-Palmitoylated Proteins.

  • Abrami L Global Health Institute, School of Life Sciences, EPFL, Lausanne, Switzerland.
  • Denhardt-Eriksson RA Global Health Institute, Laboratory of Computational Systems Biotechnology, EPFL, Lausanne, Switzerland.
  • Hatzimanikatis V Laboratory of Computational Systems Biotechnology, EPFL, Lausanne, Switzerland.
  • van der Goot FG Global Health Institute, School of Life Sciences, EPFL, Lausanne, Switzerland. gisou.vandergoot@epfl.ch.
  • 2019-06-02
Published in:
  • Methods in molecular biology (Clifton, N.J.). - 2019
Data-driven mathematical modeling
Metabolic labeling
Protein S-acyltransferase (PAT)
Pulse–chase
Radiolabeling
S-palmitoylation
Turnover
Cell Line, Transformed
Humans
Isotope Labeling
Lipoylation
Palmitic Acid
Protein Processing, Post-Translational
Sulfur Radioisotopes
English Proteins can be radiolabeled either during synthesis, typically using 35S-cysteine/methionine (35S-Cys/Met), or after synthesis, by adding a radiolabeled posttranslational modification. Here we describe how protein S-palmitoylation, and its dynamics, can be monitored by 3H-palmitate labeling and how the importance of S-palmitoylation in protein biogenesis and turnover can be investigated using 35S-Cys/Met pulse-chase metabolic labeling. Proteins frequently have multiple palmitoylation sites. The importance thereof on the design and interpretation of metabolic labeling experiments is discussed.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/226055
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