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HSV-1 Amplicon Vectors as Genetic Vaccines.
Journal article

HSV-1 Amplicon Vectors as Genetic Vaccines.

  • Meier AF Institute of Virology, Vetsuisse Faculty, University of Zurich, Zürich, Switzerland.
  • Laimbacher AS Musculoskeletal Research Unit (MSRU), Vetsuisse Faculty, University of Zurich, Zürich, Switzerland. andrea.laimbacher@uzh.ch.
  • 2019-10-17
Published in:
  • Methods in molecular biology (Clifton, N.J.). - 2020
Genetic vaccine
Helper virus-free HSV-1 amplicon vector
Polycistronic transgene cassette
VLPs
Virus-like particles
Animals
Chlorocebus aethiops
Genetic Vectors
Herpesvirus 1, Human
Humans
Mice
Transduction, Genetic
Vero Cells
Viral Structural Proteins
Viral Vaccines
English HSV-1 amplicon vectors have been used as platforms for the generation of genetic vaccines against both DNA and RNA viruses. Mice vaccinated with such vectors encoding structural proteins from both foot-and-mouth disease virus and rotavirus were partially protected from challenge with wild-type virus (D'Antuono et al., Vaccine 28:7363-7372, 2010; Laimbacher et al., Mol Ther 20:1810-1820, 2012; Meier et al., Int J Mol Sci 18:431, 2017), indicating that HSV-1 amplicon vectors are attractive tools for the development of complex and safe genetic vaccines.This chapter describes the preparation and testing of HSV-1 amplicon vectors that encode individual or multiple viral structural proteins from a polycistronic transgene cassette. We further put particular emphasis on generating virus-like particles (VLPs) in vector-infected cells. Expression of viral genes is confirmed by Western blot and immune fluorescence analysis and generation of VLPs in vector-infected cells is demonstrated by electron microscopy. Furthermore, examples on how to analyze the immune response in a mouse model and possible challenge experiments are described.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/226372
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