Journal article
Transcriptome analysis of equine sarcoids.
- Semik E Department of Genomics and Molecular Biology of Animals, National Research Institute of Animal Production, Balice, Poland.
- Gurgul A Department of Genomics and Molecular Biology of Animals, National Research Institute of Animal Production, Balice, Poland.
- Ząbek T Department of Genomics and Molecular Biology of Animals, National Research Institute of Animal Production, Balice, Poland.
- Ropka-Molik K Department of Genomics and Molecular Biology of Animals, National Research Institute of Animal Production, Balice, Poland.
- Koch C ISME - Equine Clinic Bern, Department of Clinical Veterinary Medicine, Vetsuisse Faculty, University of Bern, Bern, Switzerland.
- Mählmann K Equine Clinic, General Surgery and Radiology, Freie Universität Berlin, Berlin, Germany.
- Bugno-Poniewierska M Department of Genomics and Molecular Biology of Animals, National Research Institute of Animal Production, Balice, Poland.
- 2016-10-26
Published in:
- Veterinary and comparative oncology. - 2017
differentially expressed genes
equine sarcoids
gene expression microarray
tumorigenesis
Animals
Biomarkers
Gene Expression Profiling
Gene Expression Regulation, Neoplastic
Gene Ontology
Horse Diseases
Horses
Oligonucleotide Array Sequence Analysis
Real-Time Polymerase Chain Reaction
Skin Neoplasms
English
Equine sarcoids are the most commonly detected skin tumours in Equidae. In the present research, a comparative transcriptomic analysis was performed which aimed at looking inside a tumour biology and identification of the expression profile as a potential source of cancer specific genes useful as biomarkers. We have used Horse Gene Expression Microarray data from matched equine sarcoids and tumour-distant skin samples. In total, 901 significantly differentially expressed genes (DEGs) between lesional and healthy skin samples have been identified (fold change ≥ 2; P < 0.05). The large subset of DEGs, with decreased expression, was associated with a suppression of malignant transformation, whereas several overexpressed genes were involved in the processes associated with growth and progression of a tumour or immune system activity. Our results, as a first to date, showed comprehensive transcriptome analysis of skin tumour in horses and pinpointed significant pathways and genes related with oncogenesis processes.
- Language
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- English
- Open access status
- closed
- Identifiers
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- DOI 10.1111/vco.12279
- PMID 27779365
- Persistent URL
- https://sonar.ch/global/documents/22673
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