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Targeted Delivery of a Photosensitizer to Aggregatibacter actinomycetemcomitans Biofilm

  • Suci, Peter Center for BioInspired Nanomaterials, Montana State University, Bozeman, Montana 59717
  • Kang, Sebyung Center for BioInspired Nanomaterials, Montana State University, Bozeman, Montana 59717
  • Gmür, Rudolf Institute of Oral Biology, Section of Oral Microbiology and General Immunology, University of Zürich, Plattenstrasse 11, CH-8032 Zürich, Switzerland
  • Douglas, Trevor Center for BioInspired Nanomaterials, Montana State University, Bozeman, Montana 59717
  • Young, Mark Center for BioInspired Nanomaterials, Montana State University, Bozeman, Montana 59717
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  • Antimicrobial Agents and Chemotherapy. - American Society for Microbiology. - 2010, vol. 54, no. 6, p. 2489-2496
Pharmacology (medical)
Pharmacology
Infectious Diseases
English ABSTRACT
The ability to selectively target specific biofilm species with antimicrobials would enable control over biofilm consortium composition, with medical applications in treatment of infections on mucosal surfaces that are colonized by a mixture of beneficial and pathogenic microorganisms. We functionalized a genetically engineered multimeric protein with both a targeting moiety (biotin) and either a fluorophore or a photosensitizer (SnCe6). Biofilm microcolonies of Aggregatibacter actinomycetemcomitans, a periodontal pathogen, were targeted with the multifunctional dodecamer. Streptavidin was used to couple biotinylated dodecamer to a biotinylated anti-A. actinomycetemcomitans antibody. This modular targeting approach enabled us to increase the loading of photosensitizer onto the cells by a cycle of amplification. Scanning laser confocal microscopy was used to characterize transport of fluorescently tagged dodecamer into the microcolonies and targeting of the cells with biotin-labeled, fluorescently tagged dodecamer. Light-induced activity of the targeted photosensitizer reduced the viability of A. actinomycetemcomitans biofilm, as indicated by membrane permeability to propidium iodide. The functionalized multimeric protein promises to be a useful tool for controlling periodontal biofilm consortia and offers a modular design whereby moieties that target different species can be readily combined with the functionalized protein construct.
Language
  • English
Open access status
bronze
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https://sonar.ch/global/documents/226971
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