Journal article
Immobilization of proteins to lipid bilayers.
- Ramsden JJ Department of Biophysical Chemistry, Biozentrum, Basel, Switzerland. ramsden@urz.unibas.ch
- Bachmanova GI
- Archakov AI
- 1996-01-01
Published in:
- Biosensors & bioelectronics. - 1996
Animals
Biosensing Techniques
Lipid Bilayers
Male
Phospholipids
Proteins
Rabbits
Sensitivity and Specificity
English
Phospholipid bilayers deposited on sensor surfaces are excellent substrates for immobilizing proteins via a molecular anchor. An integrated optics sensing device was used to accurately measure the binding kinetics of proteins thus anchored. By comparing the results with measurements using proteins from which the anchor had been enzymatically removed, it was shown that the anchor accounts for essentially all the irreversible binding. The insertion of the anchor into the lipid bilayer is a spontaneous process. This method of immobilization should be widely applicable to many soluble protein molecules, to which an anchor can be attached by routine methods of molecular engineering.
- Language
-
- English
- Open access status
- closed
- Identifiers
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- DOI 10.1016/0956-5663(96)86789-3
- PMID 8729242
- Persistent URL
- https://sonar.ch/global/documents/227604
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