Journal article
Transcriptome and translatome co-evolution in mammals.
- Wang ZY Center for Molecular Biology of Heidelberg University (ZMBH), DKFZ-ZMBH Alliance, Heidelberg, Germany.
- Leushkin E Center for Molecular Biology of Heidelberg University (ZMBH), DKFZ-ZMBH Alliance, Heidelberg, Germany. e.leushkin@zmbh.uni-heidelberg.de.
- Liechti A Center for Integrative Genomics, University of Lausanne, Lausanne, Switzerland.
- Ovchinnikova S Center for Molecular Biology of Heidelberg University (ZMBH), DKFZ-ZMBH Alliance, Heidelberg, Germany.
- Mößinger K Center for Molecular Biology of Heidelberg University (ZMBH), DKFZ-ZMBH Alliance, Heidelberg, Germany.
- Brüning T Center for Molecular Biology of Heidelberg University (ZMBH), DKFZ-ZMBH Alliance, Heidelberg, Germany.
- Rummel C Center for Integrative Genomics, University of Lausanne, Lausanne, Switzerland.
- Grützner F Robinson Research Institute, School of Biological Sciences, University of Adelaide, Adelaide, South Australia, Australia.
- Cardoso-Moreira M Center for Molecular Biology of Heidelberg University (ZMBH), DKFZ-ZMBH Alliance, Heidelberg, Germany.
- Janich P Center for Integrative Genomics, University of Lausanne, Lausanne, Switzerland.
- Gatfield D Center for Integrative Genomics, University of Lausanne, Lausanne, Switzerland.
- Diagouraga B Institute of Human Genetics, Centre National de la Recherche Scientifique, Université de Montpellier, Montpellier, France.
- de Massy B Institute of Human Genetics, Centre National de la Recherche Scientifique, Université de Montpellier, Montpellier, France.
- Gill ME Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland.
- Peters AHFM Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland.
- Anders S Center for Molecular Biology of Heidelberg University (ZMBH), DKFZ-ZMBH Alliance, Heidelberg, Germany.
- Kaessmann H Center for Molecular Biology of Heidelberg University (ZMBH), DKFZ-ZMBH Alliance, Heidelberg, Germany. h.kaessmann@zmbh.uni-heidelberg.de.
- 2020-11-12
Published in:
- Nature. - 2020
English
Gene-expression programs define shared and species-specific phenotypes, but their evolution remains largely uncharacterized beyond the transcriptome layer1. Here we report an analysis of the co-evolution of translatomes and transcriptomes using ribosome-profiling and matched RNA-sequencing data for three organs (brain, liver and testis) in five mammals (human, macaque, mouse, opossum and platypus) and a bird (chicken). Our within-species analyses reveal that translational regulation is widespread in the different organs, in particular across the spermatogenic cell types of the testis. The between-species divergence in gene expression is around 20% lower at the translatome layer than at the transcriptome layer owing to extensive buffering between the expression layers, which especially preserved old, essential and housekeeping genes. Translational upregulation specifically counterbalanced global dosage reductions during the evolution of sex chromosomes and the effects of meiotic sex-chromosome inactivation during spermatogenesis. Despite the overall prevalence of buffering, some genes evolved faster at the translatome layer-potentially indicating adaptive changes in expression; testis tissue shows the highest fraction of such genes. Further analyses incorporating mass spectrometry proteomics data establish that the co-evolution of transcriptomes and translatomes is reflected at the proteome layer. Together, our work uncovers co-evolutionary patterns and associated selective forces across the expression layers, and provides a resource for understanding their interplay in mammalian organs.
- Language
-
- English
- Open access status
- closed
- Identifiers
-
- DOI 10.1038/s41586-020-2899-z
- PMID 33177713
- Persistent URL
- https://sonar.ch/global/documents/2287
Export to…
Statistics
Document views: 58
File downloads: