Back
First screening for Brachyspira hampsonii in Swiss pigs applying a new high resolution melting assay.
Journal article

First screening for Brachyspira hampsonii in Swiss pigs applying a new high resolution melting assay.

  • Scherrer S Institute of Veterinary Bacteriology, Vetsuisse Faculty, University of Zurich, Winterthurerstrasse 270, CH 8057 Zurich, Switzerland. Electronic address: simone.scherrer@vetbakt.uzh.ch.
  • Borgström A Institute of Veterinary Bacteriology, Vetsuisse Faculty, University of Zurich, Winterthurerstrasse 270, CH 8057 Zurich, Switzerland.
  • Frei D Institute of Veterinary Bacteriology, Vetsuisse Faculty, University of Zurich, Winterthurerstrasse 270, CH 8057 Zurich, Switzerland.
  • Wittenbrink MM Institute of Veterinary Bacteriology, Vetsuisse Faculty, University of Zurich, Winterthurerstrasse 270, CH 8057 Zurich, Switzerland.
  • 2016-09-08
Published in:
  • Veterinary microbiology. - 2016
Brachyspira hampsonii
High resolution melting assay
Swine dysentery
nox gene
Animals
Brachyspira
Feces
Gram-Negative Bacterial Infections
Sequence Alignment
Sequence Analysis, DNA
Swine
Swine Diseases
Switzerland
English A new High Resolution Melting (HRM) assay was developed for the rapid detection of Brachyspira (B.) hampsonii. B. hampsonii occurs in different European countries, however, until today it has not been encountered in Switzerland. Four B. hampsonii reference strains were used to develop the HRM assay: B. hampsonii clade I ATCC BAA2463 and clade II ATCC BAA2464 strain, as well as two isolated strains P280/1 from the UK and the German isolate 5369-1x/12. A conserved region of the nox gene was used to design B. hampsonii-specific primers. The HRM melting curves for the four reference strains showed reproducible difference graphs with distinct differences between the four strains based on a slight variation between the four amplicon sequences. In addition, DNA from 22 B. hampsonii strains representing four genetic B. hampsonii groups was used to validate the method. Melting temperatures in the interval between 73.1 and 74°C were obtained for all B. hampsonii strains and allow differentiating B. hampsonii from other Brachyspira species. In total 897 Swiss porcine fecal Brachyspira isolates, cultured between 2009 and 2015, were analysed by the HRM protocol. B. hampsonii was not detected among these Swiss Brachyspira isolates. In conclusion, the rapid and low-cost HRM approach allows a sensitive and specific identification of B. hampsonii.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/229105
Statistics
Document views: 25 File downloads: