Field Validation of Laboratory Tests for Clinical Diagnosis of Sheep-Associated Malignant Catarrhal Fever

Müller-Doblies, U. U.; Li, H.; Hauser, B.; Adler, H.; Ackermann, M.

doi:10.1128/jcm.36.10.2970-2972.1998

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Journal article

Field Validation of Laboratory Tests for Clinical Diagnosis of Sheep-Associated Malignant Catarrhal Fever

Müller-Doblies, U. U. Institute of Virology 1 and
Li, H. Animal Disease Research Unit, USDA-ARS, and Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 2
Hauser, B. Institute of Veterinary Pathology, 3 University of Zürich, Zürich, Switzerland, and
Adler, H. Institute of Virology 1 and
Ackermann, M. Institute of Virology 1 and

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Journal of Clinical Microbiology. - American Society for Microbiology. - 1998, vol. 36, no. 10, p. 2970-2972

Microbiology (medical)

English Until recently, sheep-associated malignant catarrhal fever (SA-MCF) was diagnosed mainly on the basis of clinical presentation and histopathological changes. Using clinically diagnosed field cases, we have evaluated a seminested PCR and a competitive inhibition enzyme-linked immunosorbent assay (CI-ELISA) and compared these assays in the diagnosis of SA-MCF in cattle with histopathology as a provisional “gold standard.” Samples from 44 cattle with clinical signs suggestive of SA-MCF were examined by histopathology, PCR, and CI-ELISA. In addition, samples from healthy cattle were evaluated by PCR (n = 96) and CI-ELISA (n = 75). Based on histopathology, 38 of the 44 clinical cases were classified as SA-MCF positive, 3 were classified as inconclusive, and 3 were classified as SA-MCF negative. The sensitivity of PCR was 95 to 97%, whereas the specificity ranged between 94 and 100%. The CI-ELISA showed a sensitivity of 56 to 87% and a specificity between 91 and 100%. In the field, there is good correlation between the diagnoses of SA-MCF by histopathology, PCR, and CI-ELISA. These data also confirm the close association of ovine herpesvirus 2 with SA-MCF in Switzerland.

Language

English

Open access status

bronze

Identifiers

DOI 10.1128/jcm.36.10.2970-2972.1998
ISSN 1098-660X

Persistent URL

https://sonar.ch/global/documents/22927

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