Journal article
Identification and implication of an allergenic PR-10 protein from walnut in birch pollen associated walnut allergy.
- Wangorsch A VPr1 Research Group Molecular Allergology, Paul-Ehrlich-Institut, Langen, Germany.
- Jamin A VPr1 Research Group Molecular Allergology, Paul-Ehrlich-Institut, Langen, Germany.
- Lidholm J ThermoFisherScientific, Uppsala, Sweden.
- Gräni N Department of Dermatology, University Hospital Zürich, Zürich, Switzerland.
- Lang C Department of Dermatology, University Hospital Zürich, Zürich, Switzerland.
- Ballmer-Weber B Department of Dermatology, University Hospital Zürich, Zürich, Switzerland.
- Vieths S VPr1 Research Group Molecular Allergology, Paul-Ehrlich-Institut, Langen, Germany.
- Scheurer S VPr1 Research Group Molecular Allergology, Paul-Ehrlich-Institut, Langen, Germany.
- 2017-01-11
Published in:
- Molecular nutrition & food research. - 2017
Bet v 1 like
Juglans regia
Jug r 5
PR-10
Walnut allergy
Allergens
Amino Acid Sequence
Antigens, Plant
Betula
Cross Reactions
Female
Histamine Release
Humans
Hypersensitivity
Immunoblotting
Immunoglobulin E
Juglans
Nuts
Plant Proteins
Pollen
English
SCOPE
English walnut (Juglans regia) belongs to the most important allergenic tree nuts. Co-sensitization with birch (Betula verrucosa) pollen has been reported. We aimed to identify a walnut allergen homologous to the major birch pollen allergen Bet v 1.
METHODS AND RESULTS
A cDNA encoding a Bet v 1-homologous allergen (Jug r 5) in walnut kernels was cloned by RT-PCR. Jug r 5 was expressed in Escherichia coli, purified by column chromatography and characterized by circular dichroism spectroscopy. Specific IgE levels to walnut, Bet v 1, and Jug r 5 in birch pollen allergics (n = 16) with concomitant walnut allergy were measured by ImmunoCAP: 44% of the patients were tested positive to walnut while 94% were reactive to Jug r 5, and 100% to Bet v 1. Jug r 5 and Bet v 1 allergens showed bidirectional IgE cross-reactivity by competitive ELISA and were capable of inducing histamine release from effector cells. Immunoblot competition experiments demonstrated the presence of IgE-reactive Jug r 5 in walnut extract, but at low levels.
CONCLUSION
A Bet v 1-like allergen was identified in walnut. Diagnostic use of Jug r 5 will compensate for the low sensitivity of walnut extract for patients with birch pollen associated walnut allergy.
English walnut (Juglans regia) belongs to the most important allergenic tree nuts. Co-sensitization with birch (Betula verrucosa) pollen has been reported. We aimed to identify a walnut allergen homologous to the major birch pollen allergen Bet v 1.
METHODS AND RESULTS
A cDNA encoding a Bet v 1-homologous allergen (Jug r 5) in walnut kernels was cloned by RT-PCR. Jug r 5 was expressed in Escherichia coli, purified by column chromatography and characterized by circular dichroism spectroscopy. Specific IgE levels to walnut, Bet v 1, and Jug r 5 in birch pollen allergics (n = 16) with concomitant walnut allergy were measured by ImmunoCAP: 44% of the patients were tested positive to walnut while 94% were reactive to Jug r 5, and 100% to Bet v 1. Jug r 5 and Bet v 1 allergens showed bidirectional IgE cross-reactivity by competitive ELISA and were capable of inducing histamine release from effector cells. Immunoblot competition experiments demonstrated the presence of IgE-reactive Jug r 5 in walnut extract, but at low levels.
CONCLUSION
A Bet v 1-like allergen was identified in walnut. Diagnostic use of Jug r 5 will compensate for the low sensitivity of walnut extract for patients with birch pollen associated walnut allergy.
- Language
-
- English
- Open access status
- closed
- Identifiers
-
- DOI 10.1002/mnfr.201600902
- PMID 28070926
- Persistent URL
- https://sonar.ch/global/documents/22999
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