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Stereoselective determination of methadone in serum by HPLC following solid-phase extraction on disk.
Journal article

Stereoselective determination of methadone in serum by HPLC following solid-phase extraction on disk.

  • Rudaz S Laboratory of Pharmaceutical Analytical Chemistry, University of Geneva, Switzerland.
  • Veuthey JL
  • 1996-06-01
Published in:
  • Journal of pharmaceutical and biomedical analysis. - 1996
Analgesics, Opioid
Chromatography, High Pressure Liquid
Heroin Dependence
Humans
Hydrogen-Ion Concentration
Methadone
Orosomucoid
Protein Binding
Reproducibility of Results
Spectrophotometry, Ultraviolet
Stereoisomerism
English A solid-phase extraction (SPE) technique for methadone has been developed using a mixed-mode solid-phase extraction disk which contains both hydrophobic and cation-exchange functional groups. The SPE technique was used to isolate the drug from the biological matrix and to prepare a cleaner sample prior to stereoselective analysis by HPLC on a silica column with covalently bound alpha 1-acid glycoprotein (Chiral-AGP) followed by ultraviolet detection at 205 mm. The within-run precision was less than 5% for the complete method over the therapeutic range. The quantification limit was 25 ng ml-1. The between-run precision was less than 15% at the quantification limit. The between-run precision at other concentrations was less than 8.5% with an accuracy of more than 95%. The mean recovery for R-methadone was 78.5% and the mean recovery for S-methadone was 73.4%. The complete procedure has been validated. This method was successfully used for the analysis of 15 clinical cases.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/230243
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