Cloning of a human ether-a-go-go potassium channel expressed in myoblasts at the onset of fusion.
- Occhiodoro T Département de Physiologie, Centre Médical Universitaire, Hôpital Cantonal Universitaire, Switzerland.
- Bernheim L
- Liu JH
- Bijlenga P
- Sinnreich M
- Bader CR
- Fischer-Lougheed J
- 1998-09-17
Published in:
- FEBS letters. - 1998
Adolescent
Adult
Amino Acid Sequence
Brain
Cell Differentiation
Cells, Cultured
Child
Child, Preschool
Cloning, Molecular
Ether-A-Go-Go Potassium Channels
Humans
Infant
Molecular Sequence Data
Muscle, Skeletal
Potassium Channels
Sequence Alignment
Sequence Analysis
English
An early sign of human myoblast commitment to fusion is the expression of a non-inactivating delayed rectifier K+ current, I(K(NI)), and an associated membrane potential hyperpolarization. We have isolated the full-length coding region of a human ether-a-go-go K+ channel (h-eag) from myoblasts undergoing differentiation. The h-eag gene was localized to chromosome 1q32-41, and is expressed as a approximately 9 kb transcript in myogenic cells and in adult brain tissue. Forced expression of h-eag in undifferentiated myoblasts generates a current with remarkable similarity to I(K(NI)) indicating that h-eag constitutes the channel responsible for this current in vivo.
- Language
-
- English
- Open access status
- bronze
- Identifiers
-
- DOI 10.1016/s0014-5793(98)00973-9
- PMID 9738473
- Persistent URL
- https://sonar.ch/global/documents/230305
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