Selection of Vibrio crassostreae relies on a plasmid expressing a type 6 secretion system cytotoxic for host immune cells.
- Piel D Unité Physiologie Fonctionnelle des Organismes Marins ZI de la Pointe du Diable, CS 10070, Ifremer, F-29280, Plouzané, France.
- Bruto M Sorbonne Universités, UPMC Paris 06, CNRS, UMR 8227, Integrative Biology of Marine Models, Station Biologique de Roscoff, CS 90074, F-29688, Roscoff cedex, France.
- James A Unité Physiologie Fonctionnelle des Organismes Marins ZI de la Pointe du Diable, CS 10070, Ifremer, F-29280, Plouzané, France.
- Labreuche Y Unité Physiologie Fonctionnelle des Organismes Marins ZI de la Pointe du Diable, CS 10070, Ifremer, F-29280, Plouzané, France.
- Lambert C Laboratoire des Sciences de l'Environnement Marin, UMR 6539 CNRS UBO IRD IFREMER, Institut Universitaire Européen de la Mer, Technopôle Brest-Iroise - Rue Dumont d'Urville, F-29280, Plouzané, France.
- Janicot A Sorbonne Universités, UPMC Paris 06, CNRS, UMR 8227, Integrative Biology of Marine Models, Station Biologique de Roscoff, CS 90074, F-29688, Roscoff cedex, France.
- Chenivesse S Sorbonne Universités, UPMC Paris 06, CNRS, UMR 8227, Integrative Biology of Marine Models, Station Biologique de Roscoff, CS 90074, F-29688, Roscoff cedex, France.
- Petton B Unité Physiologie Fonctionnelle des Organismes Marins ZI de la Pointe du Diable, CS 10070, Ifremer, F-29280, Plouzané, France.
- Wegner KM AWI - Alfred Wegener Institut, Helmholtz-Zentrum für Polar und Meeresforschung, Coastal Ecology, Wadden Sea Station Sylt, 25992, Hafenstrasse 43, List, Germany.
- Stoudmann C Laboratory of Molecular Microbiology, Global Health Institute, School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne, CH-1015, Lausanne, Switzerland.
- Blokesch M Laboratory of Molecular Microbiology, Global Health Institute, School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne, CH-1015, Lausanne, Switzerland.
- Le Roux F Unité Physiologie Fonctionnelle des Organismes Marins ZI de la Pointe du Diable, CS 10070, Ifremer, F-29280, Plouzané, France.
- 2019-08-08
Published in:
- Environmental microbiology. - 2019
English
Pacific oyster mortality syndrome affects juveniles of Crassostrea gigas oysters and threatens the sustainability of commercial and natural stocks of this species. Vibrio crassostreae (V. crassostreae) has been repeatedly isolated from diseased animals, and the majority of the strains have been demonstrated to be virulent for oysters. In this study, we showed that oyster farms exhibited a high prevalence of a virulence plasmid carried by V. crassostreae, while oysters, at an adult stage, were reservoirs of this virulent population. The pathogenicity of V. crassostreae depends on a novel transcriptional regulator, which activates the bidirectional promoter of a type 6 secretion system (T6SS) genes cluster. Both the T6SS and a second chromosomal virulence factor, r5.7, are necessary for virulence but act independently to cause haemocyte (oyster immune cell) cytotoxicity. A phylogenetically closely related T6SS was identified in V. aestuarianus and V. tapetis, which infect adult oysters and clams respectively. We propose that haemocyte cytotoxicity is a lethality trait shared by a broad range of mollusc pathogens, and we speculate that T6SS was involved in parallel evolution of pathogen for molluscs.
- Language
-
- English
- Open access status
- green
- Identifiers
-
- DOI 10.1111/1462-2920.14776
- PMID 31390475
- Persistent URL
- https://sonar.ch/global/documents/230774
Export to…
Statistics
Document views: 54
File downloads:
- Full-text: 0