Journal article
Plasmonic Tipless Pyramid Arrays for Cell Poration.
- Courvoisier S †Group of Applied Physics, Biophotonics Group, University of Geneva, 22 chemin de Pinchat, 1227 Carouge, Switzerland.
- Saklayen N ‡Department of Physics and School of Engineering and Applied Sciences, Harvard University, 9 Oxford Street, Cambridge, Massachusetts 02138, United States.
- Huber M ‡Department of Physics and School of Engineering and Applied Sciences, Harvard University, 9 Oxford Street, Cambridge, Massachusetts 02138, United States.
- Chen J ‡Department of Physics and School of Engineering and Applied Sciences, Harvard University, 9 Oxford Street, Cambridge, Massachusetts 02138, United States.
- Diebold ED ‡Department of Physics and School of Engineering and Applied Sciences, Harvard University, 9 Oxford Street, Cambridge, Massachusetts 02138, United States.
- Bonacina L †Group of Applied Physics, Biophotonics Group, University of Geneva, 22 chemin de Pinchat, 1227 Carouge, Switzerland.
- Wolf JP †Group of Applied Physics, Biophotonics Group, University of Geneva, 22 chemin de Pinchat, 1227 Carouge, Switzerland.
- Mazur E ‡Department of Physics and School of Engineering and Applied Sciences, Harvard University, 9 Oxford Street, Cambridge, Massachusetts 02138, United States.
- 2015-06-17
Published in:
- Nano letters. - 2015
Cell transfection
nanophotonics
nanostructured substrate
plasmonics
tipless pyramid
ultrafast laser
Cell Survival
Gold
HeLa Cells
Humans
Lasers
Nanostructures
Optics and Photonics
Surface Plasmon Resonance
Transfection
English
Improving the efficiency, cell survival, and throughput of methods to modify and control the genetic expression of cells is of great benefit to biology and medicine. We investigate, both computationally and experimentally, a nanostructured substrate made of tipless pyramids for plasmonic-induced transfection. By optimizing the geometrical parameters for an excitation wavelength of 800 nm, we demonstrate a 100-fold intensity enhancement of the electric near field at the cell-substrate contact area, while the low absorption typical for gold is maintained. We demonstrate that such a substrate can induce transient poration of cells by a purely optically induced process.
- Language
-
- English
- Open access status
- closed
- Identifiers
-
- DOI 10.1021/acs.nanolett.5b01697
- PMID 26079771
- Persistent URL
- https://sonar.ch/global/documents/23143
Statistics
Document views: 34
File downloads: