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Chronic administration of OB protein decreases food intake by selectively reducing meal size in male rats

  • Kahler, Andrea Institute for Animal Sciences, Swiss Federal Institute of Technology, 8092 Zurich, Switzerland;
  • Geary, Nori E. W. Bourne Laboratory, New York Hospital-Cornell Medical Center, White Plains, New York 10605; and
  • Eckel, Lisa A. E. W. Bourne Laboratory, New York Hospital-Cornell Medical Center, White Plains, New York 10605; and
  • Campfield, L. Arthur Department of Metabolic Diseases, Hoffmann-La Roche, Nutley, New Jersey 07110
  • Smith, Françoise J. Department of Metabolic Diseases, Hoffmann-La Roche, Nutley, New Jersey 07110
  • Langhans, Wolfgang Institute for Animal Sciences, Swiss Federal Institute of Technology, 8092 Zurich, Switzerland;
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  • American Journal of Physiology-Regulatory, Integrative and Comparative Physiology. - American Physiological Society. - 1998, vol. 275, no. 1, p. R180-R185
Physiology (medical)
Physiology
English The potent hypophagic effect of OB protein (OB) is well established, but the mechanism of this effect is largely unknown. We investigated the effects of chronic administration of a novel modified recombinant human OB (Mod-OB) with a prolonged half-life (>48 h) on ad libitum food intake, spontaneous meal patterns, and body weight in 24 adult, male Sprague-Dawley rats (body weight at study onset: 292 g). Single daily subcutaneous injections of Mod-OB (4 mg/kg daily) for 8 consecutive days significantly reduced ad libitum food intake compared with vehicle injections from injection day 3through postinjection day 3. Mod-OB-injected rats ate between 4.5 and 7.1 g (or 13–20%) per day less than controls, with the reduction primarily occurring during the dark period. Body weight gain was significantly decreased in response to Mod-OB from injection day 8until postinjection day 4, with a maximum difference of 24 g on postinjection day 3. The reduction of food intake by Mod-OB was mainly due to a 21–34% decrease in nocturnal spontaneous meal size. There was no significant effect of Mod-OB on nocturnal meal frequency or duration. Mod-OB also did not reliably affect the size, duration, or frequency of diurnal meals. Mod-OB-injected rats displayed no compensatory hyperphagia after the injection period. These results indicate that chronically administered OB selectively affects the mechanisms controlling meal size in male rats.
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  • English
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green
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https://sonar.ch/global/documents/231556
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