Molecular Basis of Tubulin Transport Within the Cilium by IFT74 and IFT81

Bhogaraju, Sagar; Cajanek, Lukas; Fort, Cécile; Blisnick, Thierry; Weber, Kristina; Taschner, Michael; Mizuno, Naoko; Lamla, Stefan; Bastin, Philippe; Nigg, Erich A.; Lorentzen, Esben

doi:10.1126/science.1240985

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Molecular Basis of Tubulin Transport Within the Cilium by IFT74 and IFT81

Bhogaraju, Sagar Department of Structural Cell Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, D-82152 Martinsried, Germany.
Cajanek, Lukas Biozentrum, University of Basel, Klingelbergstrasse 50/70, CH-4056 Basel, Switzerland.
Fort, Cécile Trypanosome Cell Biology Unit, Institut Pasteur and CNRS URA2581, 25 Rue du Docteur Roux, 75015 Paris, France.
Blisnick, Thierry Trypanosome Cell Biology Unit, Institut Pasteur and CNRS URA2581, 25 Rue du Docteur Roux, 75015 Paris, France.
Weber, Kristina Department of Structural Cell Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, D-82152 Martinsried, Germany.
Taschner, Michael Department of Structural Cell Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, D-82152 Martinsried, Germany.
Mizuno, Naoko Department of Structural Cell Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, D-82152 Martinsried, Germany.
Lamla, Stefan Department of Cell Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, D-82152 Martinsried, Germany.
Bastin, Philippe Trypanosome Cell Biology Unit, Institut Pasteur and CNRS URA2581, 25 Rue du Docteur Roux, 75015 Paris, France.
Nigg, Erich A. Biozentrum, University of Basel, Klingelbergstrasse 50/70, CH-4056 Basel, Switzerland.
Lorentzen, Esben Department of Structural Cell Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, D-82152 Martinsried, Germany.

2013-8-29

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Science. - American Association for the Advancement of Science (AAAS). - 2013, vol. 341, no. 6149, p. 1009-1012

Multidisciplinary

English Intraflagellar transport (IFT) of ciliary precursors such as tubulin from the cytoplasm to the ciliary tip is involved in the construction of the cilium, a hairlike organelle found on most eukaryotic cells. However, the molecular mechanisms of IFT are poorly understood. Here, we found that the two core IFT proteins IFT74 and IFT81 form a tubulin-binding module and mapped the interaction to a calponin homology domain of IFT81 and a highly basic domain in IFT74. Knockdown of IFT81 and rescue experiments with point mutants showed that tubulin binding by IFT81 was required for ciliogenesis in human cells.

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English

Open access status

green

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DOI 10.1126/science.1240985
ISSN 0036-8075

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https://sonar.ch/global/documents/231762

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